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Diego Orzaez Lab: Orzaez lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
160553Tobacco Etch Virus NIa protease (GB0420)pUPD_NIaPro(TEV)
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160554pUPD P35S (GB0552)35s promoter for N-terminal fusions
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160555pUPD Pnos (GB0555)Nos promoter for N-terminal fusions
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160556pUPD DsRed-CT (GB1183)DsRed for C-terminal fusions
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160561pVD1 tRNA-gRNA position E2 (GB2239)tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA
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160562pVD1 tRNA-gRNA position E3 (GB2240)tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
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160563pVD1 Multiplexing Edit E4 (GB2241)tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.
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160564pVD1 Multiplexing Edit En-1 (GB2242)tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
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160565pVD1 tRNA-gRNA E4-En-1 (GB2243)tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
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160566pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
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160567pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
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160569pUPD2 hpIntron (GB1281)Intron sequence from S. lycopersicum
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160584pUPD2 AlcA (GB1549)Ethanol inducible operator
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160585pUPD2 AlcR (GB1550)Ethanol dependent transcrition factor
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160587pUPD2 nickase D10A Cas9 (GB1690)Nickase D10A Cas9 for Nt and Ct fusion.
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160588pUPD2 nickase D10A Cas9 (GB1691)Nickase D10A Cas9 for Nt fusion.
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160589pUPD2 NtSPrbcS (GB2213)B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain
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160590pUPD2 GFP-NT (GB2236)GFP for N-terminal fusions
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160591pUPD2 GFP (GB2237)GFP coding sequence.
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160592pUPD2 GFP-CT (GB2238)GFP for C-terminal fusions.
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160595pUPD2_miniDFR (GB2566)Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region
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160596pUPD2 dCas9 (GB2664)CDS of dCas9 with B3 nomenclature
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160597pUPD2 MS2 (GB2665)CDS of MS2 protein with B3 nomenclature
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160598pUPD2 ER-ER (GB2666)two repeats of ER (Estradiol receptor)
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160599pUPD2 CUP2 (GB2716)Copper dependent transcription factor
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160600pUPD2 VP16 (GB2717)Transcriptional activator
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160601pUPD2 CBS4 (GB2718)Copper dependent CUP2 binding DNA sequence
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160602pUPD2 Etr4 (GB2719)
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160603pUPD2 PhyB (GB2721)chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
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160605pUPD2 SV40-AcrIIA4 (GB3333)SV40-AcrIIA4 codon optimized for N.benthamiana
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160606pEGB3 alpha1 35s:PIF6:tNos (GB0170)TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
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160607pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.
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160608pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)
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160612GB_PVX (GB1464)PVX vector adapted to GB
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160618pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
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160628pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)Module for the constitutive expression of the nptII, Cas9 and DsRed genes.
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160629pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)TU for the expression of LbCas12a
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160630pEGB3_alpha2 35:PhyB:VP16 (GB2745)TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
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160631pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
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160632pEGB3_alpha1 Pnos:P19:Tnos (GB2762)TU for expression of P19 driven by Nos promoter.
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160634pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)TU for wtGFP expression driven by 2x35s promoter.
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160635pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator
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160637pDGB3 alpha1 P35S:BFP:T35S (GB3416)TU for the constitutive expression of Blue Fluorescent Protein
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160638AtS/MAR10 insulator sequence (GB3458)pEGB3a1 AtS/MAR10
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160639AtS/MAR10 insulator sequence (GB3459)pEGB3a2 AtS/MAR10
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160640pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
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160646pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)Module for the constitutive expression of the nptII, Cas9 and DsRed genes.
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160647pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)module containing the human codon optimized Cas9 and the BASTA selection marker
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