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Diego Orzaez Lab: Orzaez lab plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
160553Tobacco Etch Virus NIa protease (GB0420)pUPD_NIaPro(TEV)
160555pUPD Pnos (GB0555)Nos promoter for N-terminal fusions
160556pUPD DsRed-CT (GB1183)DsRed for C-terminal fusions
160569pUPD2 hpIntron (GB1281)Intron sequence from S. lycopersicum
160584pUPD2 AlcA (GB1549)Ethanol inducible operator
160585pUPD2 AlcR (GB1550)Ethanol dependent transcrition factor
160587pUPD2 nickase D10A Cas9 (GB1690)Nickase D10A Cas9 for Nt and Ct fusion.
160588pUPD2 nickase D10A Cas9 (GB1691)Nickase D10A Cas9 for Nt fusion.
160589pUPD2 NtSPrbcS (GB2213)B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain
160590pUPD2 GFP-NT (GB2236)GFP for N-terminal fusions
160591pUPD2 GFP (GB2237)GFP coding sequence.
160592pUPD2 GFP-CT (GB2238)GFP for C-terminal fusions.
160596pUPD2 dCas9 (GB2664)CDS of dCas9 with B3 nomenclature
160597pUPD2 MS2 (GB2665)CDS of MS2 protein with B3 nomenclature
160598pUPD2 ER-ER (GB2666)two repeats of ER (Estradiol receptor)
160599pUPD2 CUP2 (GB2716)Copper dependent transcription factor
160600pUPD2 VP16 (GB2717)Transcriptional activator
160601pUPD2 CBS4 (GB2718)Copper dependent CUP2 binding DNA sequence
160602pUPD2 Etr4 (GB2719)
160603pUPD2 PhyB (GB2721)chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
160607pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.
160612GB_PVX (GB1464)PVX vector adapted to GB
160618pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
160629pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)TU for the expression of LbCas12a
160630pEGB3_alpha2 35:PhyB:VP16 (GB2745)TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
160631pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
160632pEGB3_alpha1 Pnos:P19:Tnos (GB2762)TU for expression of P19 driven by Nos promoter.
160634pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)TU for wtGFP expression driven by 2x35s promoter.
160637pDGB3 alpha1 P35S:BFP:T35S (GB3416)TU for the constitutive expression of Blue Fluorescent Protein
160639AtS/MAR10 insulator sequence (GB3459)pEGB3a2 AtS/MAR10
160640pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
160647pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)module containing the human codon optimized Cas9 and the BASTA selection marker

Antibodies from Article