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We narrowed to 8 results for: 68375

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Showing: 1 - 8 of 8 results

  1. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Promoter
    human PGK1
    Available Since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

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  1. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  2. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid
    #176932
    Purpose
    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK1
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    176932-plasmid-map-sequence-id-378103

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  3. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

    Plasmid
    #191527
    Purpose
    Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG) + 3xFLAG_TwinStrep
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191527-plasmid-map-sequence-id-378107

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  6. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available Since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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Showing: 1 - 8 of 8 results