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Showing: 1 - 30 of 8845 results

  1. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral Prep
    #92379-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .
    Depositor
    John Doench, David Root
    Article
    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:
    Available Since
    Aug. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  11. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  13. pRSET.HaloTag.v7

    Plasmid
    #226197
    Purpose
    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Tags
    6xHIS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226197-plasmid-map-sequence-id-449504

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  18. pRosettav2

    Plasmid
    #115557
    Purpose
    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments
    Depositor
    John Doench
    Insert
    Hygromycin-Blasticidin-Puromycin-EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115557-plasmid-map-sequence-id-226246

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  21. SETD7

    Plasmid
    #40746
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7 (SETD7 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    Nov. 14, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    40746-plasmid-map-sequence-id-364373

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  22. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. SETD1A

    Plasmid
    #32868
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD1A (SETD1A Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. SETD2

    Plasmid
    #40741
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD2 (SETD2 Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Article
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available Since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

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Showing: 1 - 30 of 8845 results