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Showing: 1 - 40 of 25909 results

  1. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  2. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  3. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.
    Depositor
    Alexis Komor
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. pVITRO1-dV-IgG1/κ

    Plasmid
    #52213
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52213-plasmid-map-sequence-id-322304

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  9. pVITRO1-dV-IgG1/λ

    Plasmid
    #52214
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52214-plasmid-map-sequence-id-432867

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  10. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Use
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  19. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

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  21. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

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  22. pETSUMO2_Vitiosangium-bGSDM

    Plasmid
    #212127
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Vitiosangium bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212127-plasmid-map-sequence-id-419098

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  24. env8 selectivity (b')

    Plasmid
    #99837
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (b')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99837-plasmid-map-sequence-id-192118

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  26. env8 selectivity (d')

    Plasmid
    #99838
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (d')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99838-plasmid-map-sequence-id-192115

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  35. pVITRO1-HAPPID1

    Plasmid
    #204626
    Purpose
    Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.
    Depositor
    James McDonnell
    Article
    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75
    Inserts
    HAPPID1 heavy chain
    HAPPID1 light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse elongation factor 1 alpha and rat elongatio…
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204626-plasmid-map-sequence-id-416168

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Showing: 1 - 40 of 25909 results