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Showing: 361 - 400 of 21949 results

  6. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Use
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60492-plasmid-map-sequence-id-96974

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  7. pJC30X-BS(X)

    Plasmid
    #58958
    Purpose
    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.
    Insert
    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    Use
    Tags
    First 8 codons of COX3
    Expression
    Mutation
    GFP modified as described: CORMACK, B. P., R. H. …
    Promoter
    n/a
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. CMV-Y-GECO1

    Plasmid
    #55766
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Use
    Tags
    6x His tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55766-plasmid-map-sequence-id-89442

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  9. pTorPE-Y-GECO1

    Plasmid
    #55765
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Use
    Lab constructed
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55765-plasmid-map-sequence-id-89444

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  14. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  19. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    75036-plasmid-map-sequence-id-142299

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  21. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VC155
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22011-plasmid-map-sequence-id-354492

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  22. pBiFC-VN173

    Plasmid
    #22010
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VN173
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Venus residus 1-172
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22010-plasmid-map-sequence-id-354487

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  23. pEG03

    Plasmid
    #154138
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154138-plasmid-map-sequence-id-332116

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  29. pEG03'

    Plasmid
    #162578
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162578-plasmid-map-sequence-id-332111

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  30. pEG01

    Plasmid
    #154136
    Purpose
    This plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_BAD
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154136-plasmid-map-sequence-id-332112

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  31. pEG02

    Plasmid
    #154137
    Purpose
    This plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_thrC3
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154137-plasmid-map-sequence-id-332114

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  38. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Mutation
    Promoter
    pdx
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

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  40. Pxyl-rsaA

    Plasmid
    #207250
    Purpose
    Integrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter region
    Depositor
    Suliana Manley
    Article
    Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:
    Insert
    rsaA (promoter to codon sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207250-plasmid-map-sequence-id-407933

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Showing: 361 - 400 of 21949 results