-
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH3proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
GammaPCDH-A3-GFP/pEGFP-N1
Plasmid#197343PurposePlasmid expressing an antigen targeting Pan-Gamma-protocadherin-ConstantDepositorInsertpcdh-gamma-A3
UseTagsGFP (GFP observed)ExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdBIP1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:RFP
Plasmid#108455PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertTol2-Amp-y-cryst-mCherry
UseGatewayTagsExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL
Plasmid#64194PurposeFluorescent reporter for ZAP-70 tyrosine kinase activityDepositorInsertROZA-XL
UseTagsExpressionMammalianMutationROZA-XLPromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEK20
Plasmid#210582PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdGAPDHproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
UseTagsmRuby3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA)
Plasmid#123150PurposeMercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
UseTagsH6ExpressionBacterialMutationNonePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET ΔPRY/SPRY
Plasmid#218516PurposeRIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1DepositorInsertRIPLET delta PRY/SPRY domain (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK21
Plasmid#210586PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdTUBA1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdTUBA1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK23
Plasmid#210595PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH4pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH4proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK24
Plasmid#210597PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdACTINpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdACTINproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorInsertDrp1 (Drp1 Fly)
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-CDH1 wtUTR
Plasmid#25038DepositorInsertCDH1 3'UTR (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-2
Plasmid#42102DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11)
Plasmid#123145PurposeArsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL YF
Plasmid#64195PurposeFluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequenceDepositorInsertROZA-XL YF
UseTagsExpressionMammalianMutationROZA-XL tyrosine phosphorylation site mutated to …PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1+2
Plasmid#42106DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-2
Plasmid#42105DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-1+2
Plasmid#42103DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1
Plasmid#42104DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-1
Plasmid#42101DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
ECibN
Plasmid#209670PurposeExpresses E-cadherin fused with eGFP, CIB1, and Split-TurboID-N in mammalian cellsDepositorInsertE-Cadherin (CDH1 Canis lupus familiaris)
UseTagsCIB1, Split-TurboID-N, and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-HsPcdh21-mNeonGreen
Plasmid#210819PurposeMammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)DepositorInsertProtocadherin 21 (CDHR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP- SCAT3.2
Plasmid#172497PurposeSCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.DepositorInsertAmcyan
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterMLV LTRAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
loxP-myctagBFP-STOP-loxP-mCherry-NTR
Plasmid#108456PurposeThis plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BACDepositorInsertloxP-myctagBFP-STOP-loxP-mCherry-NTR
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIR-CDH1 mutUTR
Plasmid#25039DepositorInsertCDH1 mutant 3'UTR (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationsubstitution of 6nt within the miR-9 binding site…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
hMEF2A K403R
Plasmid#118355PurposeThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.DepositorInserthMEF2A (MEF2A Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK403RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His-mPer1
Plasmid#219421PurposeExpresses 3873 bp of mPer1 coding sequence in E. coli.DepositorInsertmPer1 (Per1 Mouse)
UseTagsHis and V5ExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-HsPcdh21-Myc-Flag-His
Plasmid#210818PurposeMammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)DepositorInsertProtocadherin 21 (CDHR1 Human)
UseTagsMyc, 3x Flag, 10x HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(g8)
Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1-Armc12/FLAG
Plasmid#180494PurposeExpression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1-Armc12 delta ARM/FLAG
Plasmid#180496PurposeExpression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationARM domain of Armc12 is deletedPromoterCAG promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Armc12/FLAG/BioID2/HA
Plasmid#180498PurposeExpression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1-Armc12 delta N/FLAG
Plasmid#180495PurposeExpression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationN-terminus of Armc12 is deletedPromoterCAG promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1-Armc12 delta C/FLAG
Plasmid#180497PurposeExpression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationC-terminus of Armc12 is deletedPromoterCAG promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rescue myc-PCDHGC5 mRNA
Plasmid#122229PurposeRescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
UseTags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationSilent mutations are in five consecutive codons …PromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-mCherry-TOSI
Plasmid#172496PurposemCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPDCD4 (Pdcd4 Mouse)
UseRetroviralTagsMcherryExpressionMammalianMutationPromoterMLV LTRAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-mVenus-TOSI
Plasmid#172491PurposemVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPdcd4 (Pdcd4 Mouse)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterMLV LTRAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only