Skip to main content
Addgene
Showing: 181 - 200 of 2024 results

  6. pOPINF Ta-sro1 WWE-PARP

    Plasmid
    #192550
    Purpose
    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)
    Depositor
    Lennart Wirthmueller
    Article
    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d
    Insert
    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    Use
    Tags
    His6, 3C protease cleavage site
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    T7 promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    192550-plasmid-map-sequence-id-378819

    Enlarge

  7. Anti-TARPGamma2/4/8 [N245/36R-2b]

    Plasmid
    #220426
    Purpose
    Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N245/36.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Tarp Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220426-plasmid-map-sequence-id-435337

    Enlarge

  9. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

    Enlarge

  12. pENTR4 CDS A. thaliana PARP1

    Plasmid
    #175818
    Purpose
    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Insert
    PARP1 (PARP1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    one silent mutation, annotated in GenBank file
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175818-plasmid-map-sequence-id-346172

    Enlarge

  17. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Use
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

    Enlarge

  18. WARP-COMP-blac-flag-his

    Plasmid
    #110989
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    von Willebrand factor A-domain related protein (WARP) (PF08_0136b Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110989-plasmid-map-sequence-id-224479

    Enlarge

  20. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Joan Conaway
    Article
    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    175949-plasmid-map-sequence-id-345352

    Enlarge

Showing: 181 - 200 of 2024 results