We narrowed to 318 results for: GCN4

1. pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

   Plasmid

   #60909

   Purpose

   A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   K560-E236A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PGK-scFv GCN4-sfGFP-VP64

   Plasmid

   #122133

   Purpose

   Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   scFv GCN4-sfGFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4

   Plasmid

   #107310

   Purpose

   Encoding dCas9-10xGCN4 driven by dSV40 promoter

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   dSV40-dCas9-10xGCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

   Plasmid

   #100944

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   F636A, E660A, E725A, R295A

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPI4-Strep-SNAP-CD28-GCN4-His6

   Plasmid

   #223585

   Purpose

   Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

   Plasmid

   #223582

   Purpose

   Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

   Plasmid

   #60906

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa

   Plasmid

   #221354

   Purpose

   Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE

    Plasmid

    #106303

    Purpose

    The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    scFv-GCN4-HaloTag-GB1-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

    Plasmid

    #175020

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    s57a esterase knock out mutant

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175019

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

    Plasmid

    #120555

    Purpose

    Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    Cas9, NES, ERT2 and GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

    Plasmid

    #186745

    Purpose

    dCas9-10XGcn4-P2A-mCherry expression construct

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    Cas9-10XGcn4-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

    Plasmid

    #174632

    Purpose

    Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid

    #121966

    Purpose

    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    superfold GFP-GB1-NLS-iLID

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174640

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174643

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    

    Use

    Tags

    SSPB(nano)-GFP

    Expression

    Mammalian

    Mutation

    EGFP: Met1Del; ppKin14VIb(aa861-1321)

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge