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Plasmid#137813Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v3 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region, N280D in the V3 regionPromoterPGKAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v9 (-cyt)
Plasmid#137819Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v9 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Pl-B
Plasmid#79161PurposeExpresses the Pl-B biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Pl-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(delta JBD)
Plasmid#51767Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 126-263PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-272)
Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-151)
Plasmid#58926PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-151aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-212)
Plasmid#58927PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-212aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-His-Arp11(1–417)
Plasmid#51400Purposeexpression of full-length mouse Arp11 under T7 promoterDepositorInsertArp11 (Actr10 Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-STReTCh-RGD
Plasmid#216532PurposeExpresses HaloTag-STReTCh-RGD fusion protein in bacteriaDepositorInsertHaloTag-STReTCh-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T7 Empty target Tp901
Plasmid#195885PurposeLevel 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.DepositorInsertEmpty Tp901 Target
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-TetO-QF_T7-LacO-TetR_p15A
Plasmid#171659PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.DepositorInsertT7-LacO-TetR-T7 Stop
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-TetO-GFP_ColE1
Plasmid#171662PurposeQUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.DepositorInsertQUAS-T7-TetO-GFP ssrA-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
UseTagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
UseTagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 T7 Jip1b(471-707)
Plasmid#52428Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470PromoterAvailable sinceApril 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-470)
Plasmid#52125Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281 and 471-707PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-707)
Plasmid#52126Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-470)
Plasmid#51724Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 471-660PromoterAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only