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Plasmid#204161PurposepRegev_S1 with double mutations that restore secondary structure.DepositorInsertS1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>A and 46G>U mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G.51A>C
Plasmid#204165PurposepRegev_S2 with double mutations that restore secondary structure.DepositorInsertExon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A.59G>U
Plasmid#204177PurposepRegev_S5 with double mutations that restore secondary structure.DepositorInsertExon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A and 59G>U mutationsPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-BGG-PTC_E
Plasmid#146191PurposeMammalian Expression of BGG-PTCDepositorInsertBGG-PTC (HBB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-BGG-WT_E
Plasmid#146192PurposeMammalian Expression of BGG-WTDepositorInsertBGG-WT (HBB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc
Plasmid#198113PurposeExpress hCOL4A5 in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_R1683X
Plasmid#198124PurposeExpress hCOL4A5_R1683X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationR1683XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS:bloSwitch
Plasmid#108355PurposeUAS driven green to red switch reporter for B3 recombinase expressionDepositorInsertUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
UseZebrafish expressionTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_S1632X
Plasmid#198122PurposeExpress hCOL4A5_S1632X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationS1632XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_Q1016X
Plasmid#198119PurposeExpress hCOL4A5_Q1016X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationQ1016XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_G5X
Plasmid#198114PurposeExpress hCOL4A5_G5X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationG5XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_C29X
Plasmid#198115PurposeExpress hCOL4A5_C29X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationC29XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_E130X
Plasmid#198117PurposeExpress hCOL4A5_E130X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationE130XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_S36X
Plasmid#198116PurposeExpress hCOL4A5_S36X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationS36XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc_S1661X
Plasmid#198123PurposeExpress hCOL4A5_S1661X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationS1661XPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (AAV PHP.eB)
Viral Prep#164450-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (#164450). In addition to the viral particles, you will also receive purified AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) plasmid DNA. minBetaGlobin-driven expression of Cre in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterminBetaGlobinTagsNoneAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK4_003
Plasmid#123812PurposeEncodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertrglb pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJP-HBB-nLuc
Plasmid#175431PurposeExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.DepositorInsertnLUC plus 5' HBB leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-eGFP
Plasmid#193023PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only