We narrowed to 1,406 results for: transfection

1. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-rat Fcepsilon RI gamma

   Plasmid

   #8375

   Depositor

   Jean Kinet

   Article

   Blank et al Nature 1989 Jan 12;337(6203):187-9.

   Insert

   rat Fc epsilon RI gamma cDNA (Fcer1g Rat)
   

   Use

   Beta-gal

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2005

   Availability

   Academic Institutions and Nonprofits only

3. YFP-cCGNL1-Myc

   Plasmid

   #236513

   Purpose

   For expression of Paracingulin (dog) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Paschoud et al Mol Membr Biol. 2008 Jan;25(1):1-13. doi

   Insert

   CGNL1 (CGNL1 Canis lupus familiaris (dog))
   

   Use

   Tags

   Myc and YFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAHW/Joseph2-Multitagged Plasmid

   Plasmid

   #226725

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pAHW/Joseph2
   

   Use

   Drosophila s2 cell expression

   Tags

   HA, Myc, GFP, Flag, V5, 6xHis

   Expression

   Insect

   Mutation

   Promoter

   Act5C

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Clim-DC2-lifeact-mCherry

   Plasmid

   #129558

   Purpose

   pClimDCLifeAct:mCh actin filaments marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   lifeAct fused to mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMYH9-D1424H-FLAG

   Plasmid

   #183513

   Purpose

   Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Aspartic acid 1424 to Histidine

   Promoter

   CMV

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMYH9-WT-V5

   Plasmid

   #183512

   Purpose

   Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLacO-pEF1α-RFP

   Plasmid

   #179507

   Purpose

   Expresses monomeric RFP in mammalian cells under a EF1α promoter

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   monomeric RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-IP3KA

   Plasmid

   #133582

   Purpose

   Expresses IP3KA GFP tagged

   Depositor

   Christophe Erneux

   Article

   Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

   Insert

   Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

10. GFP-IP3KB

    Plasmid

    #32528

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KB (ITPKB Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGAPDH::H2B-mScarlet

    Plasmid

    #186764

    Purpose

    A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    H2B-mScarlet
    

    Use

    Overexpression

    Tags

    H2B

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-IP3KA

    Plasmid

    #32529

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KA (ITPKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pXPE

    Plasmid

    #15284

    Depositor

    Ron Vale

    Article

    Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

    Insert

    ubiquitous kinesin heavy chain (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The insert is missing the 1st 180 bp of 5' u…

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. GFP-IP3KC

    Plasmid

    #134603

    Purpose

    Expresses IP3KC GFP tagged

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KC (ITPKC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHT7::RPL15::sNluc2

    Plasmid

    #186763

    Purpose

    An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-)-V5.hOr47a

    Plasmid

    #126474

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid

    #126473

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge