We narrowed to 16,660 results for: She

1. CYLBL_Neo_CAGdCas9-KRAB

   Plasmid

   #220439

   Purpose

   CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

   Depositor

   Yin Shen

   Article

   Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

   Insert

   dCas9
   

   Use

   CRISPR and TALEN

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-MDM4

   Plasmid

   #195140

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6p1-GST-ArcΔCTD

   Plasmid

   #119878

   Purpose

   Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of nt 832-1110

   Promoter

   tac

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. alpha actinin delta ABD-GFP

   Plasmid

   #66935

   Purpose

   Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

   Depositor

   Michael Sheetz, Pere Roca-Cusachs

   Article

   Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Alpha-actinin 1 (ACTN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   actin binding domain (amino acids 30–253) is dele…

   Promoter

   CMV

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 61426-hU6-shmFmr1-EF1a-mCherry

   Plasmid

   #157859

   Purpose

   Expresses a shRNA targeting mouse Fmr1 gene

   Depositor

   Xinyu Zhao

   Article

   Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Plasmid

   #159577

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Plasmid

   #159578

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH-Csy4-T2A-SaRFN

   Plasmid

   #85755

   Purpose

   Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-SadCas9
   

   Use

   CRISPR

   Tags

   3xHA tag, Csy4, and FokI fusion via 36 amino acid…

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-Flag-Ago2-FDM3

    Plasmid

    #72210

    Purpose

    Mammalian expression of aa 227-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-226

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5-Flag-Ago2-FDM1

    Plasmid

    #72208

    Purpose

    Mammalian expression of aa 1-372 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 373-860

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5-Flag-Ago2-FDM2

    Plasmid

    #72209

    Purpose

    Mammalian expression of aa 1-517 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 518-860

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCMV5-Flag-Ago2-FDM4

    Plasmid

    #72211

    Purpose

    Mammalian expression of aa 373-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-372

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCMV5-Flag-Ago2-FDM5

    Plasmid

    #72212

    Purpose

    Mammalian expression of aa 518-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-517

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

15. MSCV-NSD3-short del(280-342)

    Plasmid

    #72550

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    280-342 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-NSD3-short del(384-645)

    Plasmid

    #72549

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    384-645 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-NSD3-short del(100-263)

    Plasmid

    #72548

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3xFlag and SV40 NLS

    Expression

    Mammalian

    Mutation

    100-263 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-TetOne-Puro-BCL9

    Plasmid

    #202662

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    BCL9 transcription coactivator (BCL9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

    Plasmid

    #198851

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND1-W86-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

    Plasmid

    #198861

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND6-W82-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge