Addgene

We narrowed to 9,116 results for: pho

Showing: 1981 - 2000 of 9116 results

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pcDNA-HA-CYLD

Plasmid

#27547

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Promoter

Available since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
pGEX Aps-SH2

Plasmid

#46404

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Aps (SH2B2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 404-523

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-deltaHeat

Plasmid

#25068

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-4SA(S164)

Plasmid

#33083

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S61A, S109A, S127A, S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBSSK p85 alpha (delta BH)

Plasmid

#13431

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta BH) (PIK3R1 Human)

Use

Tags

FLAG

Expression

Bacterial

Mutation

BH domain replaced by flag tag.

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A/T450A/S454A

Plasmid

#19845

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pRC/CMV-TYK2V362F-VSV

Plasmid

#139345

Purpose

Expression of TYK2 V362F protein (rs2304256)

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Akt(R25C)

Plasmid

#39532

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

R25C

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pUAST-attB-Flag-dTSC2 WT

Plasmid

#111807

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (gig Fly)

Use

Tags

3xFlag

Expression

Insect

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lentiviral CaMKKbeta K193A

Plasmid

#33323

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mutation

K194A

Promoter

human PGK

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag Jnk1a1(APY)

Plasmid

#15742

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(TPF) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Thr-190Ala

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pBICI_2_strong

Plasmid

#155168

Purpose

see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat4-SH2

Plasmid

#46514

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat4 (STAT4 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 133-678

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Slap-SH2

Plasmid

#46500

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap (SLA Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 61-221

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Flag Jnk1a1(TPF)

Plasmid

#15741

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(APY) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Tyr-192Phe

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pGEX Syk(C)-SH2

Plasmid

#46519

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(C) (SYK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 149-263

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Use

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
sspa-GCaMP6f

Plasmid

#67561

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-HA-CaMKKbeta rat FL WT

Plasmid

#33321

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

S3V, G96R, and P108L

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
spa-GCaMP6f

Plasmid

#67558

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only