We narrowed to 21,018 results for: cdna

1. pcDNA3-endo-EKAR4

   Plasmid

   #205513

   Purpose

   Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-EKAR4
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-mTagBFP2-MRTF-A

   Plasmid

   #182061

   Purpose

   Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mTagBFP2-MRTF-A (MRTFA Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Neo-LUC

   Plasmid

   #214368

   Purpose

   Expression of the firefly luciferase Protein

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3_V5-APEX2-HMOX1

   Plasmid

   #85824

   Purpose

   Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ExRai-AktAR

   Plasmid

   #118462

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 His-V5 Myc

   Plasmid

   #176049

   Purpose

   Expresses Myc tagged with His and V5 in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA6-N-3XFLAG-Gsk3b

   Plasmid

   #123592

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   glycogen synthase kinase 3 beta (Gsk3b Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3 Flag MKK7B2Jnk1a1

    Plasmid

    #19726

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNAintron-CDC50A-c3xFLAG

    Plasmid

    #215702

    Purpose

    Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CDC50A (TMEM30A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Tiam1-pMag(3x)-iRFP

    Plasmid

    #67305

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Tiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3/hArf5(T31N)-HA

    Plasmid

    #79429

    Purpose

    Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/hArf5(Q71L)-HA

    Plasmid

    #79428

    Purpose

    Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Flag-Cortactin

    Plasmid

    #74476

    Purpose

    This plasmid was constructed to study cortactin interactions

    Depositor

    J Thomas Parsons

    Article

    Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

    Insert

    Cortactin (Cttn Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge