We narrowed to 34,053 results for: des.2

1. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

   Plasmid

   #208388

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RP1B FUS 1-163 QQ4xSS #2

   Plasmid

   #127195

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 QQ4xSS #2 (FUS Human)
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 SARS-CoV-2 S-frag5_nostop

   Plasmid

   #153177

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag5
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDONR221 SARS-CoV-2 S-frag3_nostop

   Plasmid

   #153175

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag3
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR221 SARS-CoV-2 S-frag4_nostop

   Plasmid

   #153176

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag4
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR221 SARS-CoV-2 S-frag1_nostop

   Plasmid

   #153173

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag1
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

   Plasmid

   #169166

   Purpose

   Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

   Insert

   3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
   

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 SARS-CoV-2 S-frag6_nostop

   Plasmid

   #153184

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag6
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221 SARS-CoV-2 S-frag2_nostop

   Plasmid

   #153174

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag2
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3-bio-myc-mCE 2-210

    Plasmid

    #112715

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-Rem2 scFv [N324/2 scFv]

    Plasmid

    #219491

    Purpose

    Mammalian Expression Plasmid of anti-Rem2 (Rat) scFv. Derived from hybridoma N324/2.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Rem2 (Rattus norvegicus) recombinant mouse scFv. (Rem2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W

    Plasmid

    #211990

    Purpose

    Express gRNA against TRIM25 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting TRIM25 (TRIM25 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(NR5A2_5-2)-PGKpuroBFP-W

    Plasmid

    #211976

    Purpose

    Express gRNA against NR5A2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting NR5A2 (NR5A2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-guide-puro mBltp1 (KIAA1109) - 2

    Plasmid

    #198490

    Purpose

    lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mBltp1 (4932438A13Rik Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCMV-Blast mLST8-Flag Mut1-2

    Plasmid

    #124918

    Purpose

    For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LSB-hsa-miR-219a-2-3p

    Plasmid

    #103359

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-219a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-219a-2-3p target (MIR219A2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LSB-hsa-miR-128-2-5p

    Plasmid

    #103199

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-128-2-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-128-2-5p target (MIR128-2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-376a-2-5p

    Plasmid

    #103496

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-376a-2-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-376a-2-5p target (MIR375 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-1185-2-3p

    Plasmid

    #103181

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-1185-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-1185-2-3p target (MIR1185-2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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