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pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKIIa-Flpo

Plasmid

#183535

Purpose

INTRSECT

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Has Service

AAV8

Insert

Flpo

Use

AAV

Mutation

None

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2

Plasmid

#244088

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mem).iGlucoSnFR2

Plasmid

#244077

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-TBK1

Plasmid

#199205

Purpose

Used for the expression of TBK1 (SMC Internal No. 1996)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mRuby3

Plasmid

#244106

Purpose

Bacterial expression of green glucose sensor with inert mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Tags

mRuby3

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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TDP43-NTD

Plasmid

#241024

Purpose

Bacterial expression of TDP-43 NTD

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP43-NTD

Tags

6x His

Expression

Bacterial

Mutation

changed N81 to stop codon

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

Plasmid

#176541

Purpose

human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB omegaRNA scaffold

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6**)-mCherry

Plasmid

#168049

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101** and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-mRuby3-FTH1

Plasmid

#192804

Purpose

Stable expression of mRuby3-FTH1 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

FTH1 (FTH1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101* and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAS133

Plasmid

#211504

Purpose

AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mTagBF2

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS132

Plasmid

#211503

Purpose

AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mCherry

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN HA-NDP52

Plasmid

#199307

Purpose

For expression of the mammalian NDP52 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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