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Showing: 2021 - 2040 of 16067 results

  1. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111848-plasmid-map-sequence-id-213454

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  2. pAAV-Ef1a-Con/Foff 2.0-GCaMP6F (AAV8)

    Viral prep
    #137123-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-GCaMP6F (#137123). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-GCaMP6F plasmid DNA. EF1a-driven, Cre-dependent expression of GCaMP6f (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

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  8. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Publication
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

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  9. pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral prep
    #137139-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (#137139). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChR2(E123T/T159C)-EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre and Flp-dependent)
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP (AAV8)

    Viral prep
    #137150-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP (#137150). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of Arch3.3-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Flp-dependent)
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Publication
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58540-plasmid-map-sequence-id-90629

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  17. pAAV-Ef1a-Con/Fon-BFP (AAV8)

    Viral prep
    #137129-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-BFP (#137129). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-BFP plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of BFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    BFP (Cre and Flp-dependent)
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. pFS462

    Plasmid
    #89065
    Purpose
    his7 integration plasmid with Z3EVpr:GFP
    Depositor
    Nick Rhind
    Publication
    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    Use
    S. pombe his7 integration vector
    Promoter
    Z3EV
    Available since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89065-plasmid-map-sequence-id-181187

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  19. pAAV-nEF-Coff/Fon-iC++-EYFP (AAV8)

    Viral prep
    #137157-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-iC++-EYFP (#137157). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-iC++-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Flp-dependent)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2021 - 2040 of 16067 results