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pFLAG-YAP1

Plasmid

#66853

Purpose

Express FLAG-tagged YAP1 in mammalian cells

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

YAP1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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GFP-RB FL

Plasmid

#16004

Depositor

Olimpia Meucci

Publication

Khan et al Cell Death Differ. 2008 Oct . 15(10):166

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rb (RB1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GFP-RB FL

Plasmid

#16004

Depositor

Olimpia Meucci

Publication

Khan et al Cell Death Differ. 2008 Oct . 15(10):166

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rb (RB1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STAT1 Y701F (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCSII-EF/mt-(n1)StayGold

Plasmid

#185823

Purpose

A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Publication

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

mt-(n1)StayGold

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)

Viral prep

#100851-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Promoter

Syn

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only
USO hisB- pyrF- rpoZ-

Bacterial strain

#18049

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Bacterial resistance

Tetracycline

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
MEK1-GFP

Plasmid

#14746

Depositor

Rony Seger

Publication

Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MEK1 (MAP2K1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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MEK1-GFP

Plasmid

#14746

Depositor

Rony Seger

Publication

Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MEK1 (MAP2K1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pKD4

Plasmid

#45605

Depositor

Barry L. Wanner

Publication

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-kan-FRT

Expression

Bacterial

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Publication

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has service

AAV1, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

4 Data Hub Reports

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has service

AAV1, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

4 Data Hub Reports

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