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pRK5-HA-SZT2 2241-end

Plasmid

#87040

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3B09

Plasmid

#70998

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb87F (Hrb87F Fly)

Use

Gateway Cloning

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
MET FLAG46QDProCFPp303

Plasmid

#15589

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL HA25Q+ProCFPp303

Plasmid

#15570

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAG219 RIPK4 3'UTR mut

Plasmid

#12049

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N20 3'UTR mut (RIPK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
myc-EAF pBacPAK8

Plasmid

#15324

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pchBA-meGFP-HALO-OMP25(MTS)

Plasmid

#188663

Purpose

HaloTag-meGFP(A206K) fusion targeted to mitochondria with OMP25 targeting sequence.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

HALO-meGFP-OMP25(MTS)

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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p4nmTriangle

Plasmid

#250946

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 4nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

4nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p20nmTriangle

Plasmid

#250947

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 20nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

20nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p60nmTriangle

Plasmid

#250948

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 60nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

60nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet::MGYFPDa829/MGYFPDa829I

Plasmid

#248877

Purpose

Expression of MGYFPDa829 and its immunity protein in bacterial cells for protein purification

Depositor

Brian Liau

Article

Roh et al Nat Methods. 2025 Oct;22(10):2083-2093.

Insert

6His-MGYFPDa829, MGYFPDa829I

Expression

Bacterial

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet::dddA11-MBP/dddI

Plasmid

#248876

Purpose

Expression of DddA11-MBP and its immunity protein in bacterial cells for protein purification

Depositor

Brian Liau

Article

Roh et al Nat Methods. 2025 Oct;22(10):2083-2093.

Insert

6His-DddA11-TEV-GSlinker-MBP, DddI

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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LpABCB1-NmCherry

Plasmid

#232276

Purpose

Vector for overexpression of Lytechinus pictus ABCB1 with an N terminal mCherry tag

Depositor

Amro Hamdoun

Article

Vyas et al Development. 2022 Jun 1;149(11):dev20064

Insert

ABCB1

Use

Synthetic Biology

Tags

mCherry

Promoter

SP6

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-HyPerFLEX-UnaG-WPRE

Plasmid

#217565

Purpose

Mammalian expression of HyPerFLEX fused with UnaG

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NES-WPRE

Plasmid

#217562

Purpose

Mammalian expression of HyPerFLEX targeted to the cytoplasm

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NES

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJL403

Plasmid

#243721

Purpose

Stably expresses a chimeric reporter with a mCherry (1-105aa)-XTEN250 linker-TBRG4(351aa-631aa), flanked by 5' and 3'UTRs of ALDH18A1 and no mitochondrial targeting sequence

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

mCherry(1-105aa)-XTEN250-TBRG4(351aa-631aa), flanked by 5' and 3'UTRs of ALDH18A1 and no mitochondrial targeting sequence

Use

Lentiviral

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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