Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,548 results for: EFS

Clear keywords
Showing: 2041 - 2060 of 2548 results

  1. LSB-hsa-miR-1185-2-3p

    Plasmid
    #103181
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-1185-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-1185-2-3p target (MIR1185-2 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103181-plasmid-map-sequence-id-195411

    Enlarge

  2. LSB-hsa-miR-10a-5p

    Plasmid
    #103177
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-10a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-10a-5p target (MIR10A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103177-plasmid-map-sequence-id-208784

    Enlarge

  3. LSB-hsa-miR-10b-3p

    Plasmid
    #103178
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-10b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-10b-3p target (MIR10B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103178-plasmid-map-sequence-id-208785

    Enlarge

  4. LSB-hsa-miR-10b-5p

    Plasmid
    #103179
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-10b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-10b-5p target (MIR10B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103179-plasmid-map-sequence-id-208788

    Enlarge

  5. LSB-hsa-let-7g-3p

    Plasmid
    #103157
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7g-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7g-3p target (MIRLET7G Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103157-plasmid-map-sequence-id-213247

    Enlarge

  6. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep
    #98747-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep
    #98747-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

    Enlarge

  18. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

    Enlarge

Showing: 2041 - 2060 of 2548 results