We narrowed to 11,169 results for: ENA

1. pJJB1439

   Plasmid

   #218589

   Purpose

   Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   GES
   

   Use

   Tags

   ePTS1

   Expression

   Yeast

   Mutation

   Erg20(F96W, N127W)

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJJB170

   Plasmid

   #218591

   Purpose

   Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Adr1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJJB1340

   Plasmid

   #218593

   Purpose

   Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Pex22(1-36)

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSKT001

   Plasmid

   #218594

   Purpose

   Express degron assay: Degradation tag-YFP-ePTS1

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   YFP
   

   Use

   Tags

   UbiY degradation tag and ePTS1

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJJB1357

   Plasmid

   #218595

   Purpose

   Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex11
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKS1461

   Plasmid

   #218587

   Purpose

   Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Erg12
   

   Use

   Tags

   ePTS1

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MDH1_KW

   Plasmid

   #224145

   Purpose

   IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

   Depositor

   Katherine Walstrom

   Article

   Thomas et al Biochim Biophys Acta Proteins Proteom. 2

   Insert

   MDH-1 (F46E10.10 Nematode)
   

   Use

   Tags

   Ala-intein-CBD

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKJ248

   Plasmid

   #217494

   Purpose

   Mammlian expression of DpFnuc guide RNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   DpFnuc guide RNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_PA1472_Cterm_His

   Plasmid

   #220485

   Purpose

   heterologous expression of PA1472 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA1472
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_speG_Nterm_His

    Plasmid

    #220478

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_PA4114_Nterm_His

    Plasmid

    #220479

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_PA1472_Nterm_His

    Plasmid

    #220480

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_PA1377_Nterm_His

    Plasmid

    #220481

    Purpose

    heterologous expression of PA1377 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1377
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-H2-18 Light chain

    Plasmid

    #216308

    Purpose

    Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAN052

    Plasmid

    #220046

    Purpose

    Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    FLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKJ234

    Plasmid

    #217492

    Purpose

    Mammlian expression of DpFnuc for genome editing

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    DpFnuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xABRE_A

    Plasmid

    #185803

    Purpose

    A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

    Depositor

    Jose Dinneny

    Article

    Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

    Insert

    6xABRE(ABI1)+-86MP+erGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDV02

    Plasmid

    #212156

    Purpose

    Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Article

    Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

    Insert

    secondary alcohol dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xABRE_R

    Plasmid

    #185804

    Purpose

    a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

    Depositor

    Jose Dinneny

    Article

    Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

    Insert

    6xABRE(RD29A)+-86MP+erGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge