We narrowed to 24,831 results for: nes

1. pGEX-KG Dach2 (CT#558)

   Plasmid

   #13874

   Depositor

   Cliff Tabin

   Article

   Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

   Insert

   Dach2 (DACH2 Chicken)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pMJ922

   Plasmid

   #78312

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-EGFP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Ub KO

   Plasmid

   #11934

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin KO (RBX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   All 7 lysines of ubiquitin mutated to arginines.

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpCas9(H840A)-T2A-EGFP

   Plasmid

   #221231

   Purpose

   Express nCas9 nickase (H840A) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(H840A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.M6PR-GFP

   Plasmid

   #224407

   Purpose

   Mammalian expression of M6PR

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   M6PR (M6PR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-CRM197-4xDQNAT

   Plasmid

   #128403

   Purpose

   Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215327

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   MUC5AC Promoter
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MUC5AC

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmGFP-ADAR2

   Plasmid

   #117929

   Purpose

   ADAR2 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR2 (ADARB1 Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa.MARK2-Halo

    Plasmid

    #224414

    Purpose

    Mammalian expression of MARK2

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    MARK2 (MARK2 Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRN3P-HA-Suv4-20h1

    Plasmid

    #86689

    Purpose

    For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mutation

    Promoter

    T3

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Jason Peters, Carol Gross, Stanley Qi

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Jason Peters, Carol Gross, Stanley Qi

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215328

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    FOXJ1 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXJ1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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