We narrowed to 15,156 results for: pET

1. pET28-a(+)-6xHis-LSSmCherry1(-13)

   Plasmid

   #219415

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   LSSmCherry1(-13)
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-NAD-cpLuc2

   Plasmid

   #135629

   Purpose

   Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

   Depositor

   Kai Johnsson

   Article

   Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

   Insert

   NAD-cpLuc2 sensor
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23-CBD-(-PP)-EGFP

   Plasmid

   #12597

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   Cdc42 Biosensor (WAS Human)
   

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   F271C

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET26-Ufd1-His

   Plasmid

   #21266

   Depositor

   Hemmo Meyer

   Article

   Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

   Insert

   Ufd1 (Ufd1l Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30b jGCaMP8f F366H

   Plasmid

   #204139

   Purpose

   Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   T7

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-pro-sumo2

   Plasmid

   #25102

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO2 (SUMO2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET29b-Ava3858-3856

   Plasmid

   #45745

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3856
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pET-51b(+)-Caprola_09

    Plasmid

    #194670

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-2-PHGDH-C234S

    Plasmid

    #107724

    Purpose

    Expression of PHGDH C234S in bacteria

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    PHGDH (PHGDH Human)
    

    Expression

    Bacterial

    Mutation

    C234S

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-Ypet-PBD

    Plasmid

    #24533

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Retroviral

    Tags

    Ypet

    Expression

    Mammalian

    Available Since

    Aug. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pET42a-lambdaN+-L+-GSH

    Plasmid

    #98894

    Purpose

    Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

    Depositor

    Pascale Legault

    Article

    Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

    Insert

    lambdaN+-L+
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-iLOV-Rsn2

    Plasmid

    #61687

    Purpose

    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

    Depositor

    Brian Smith

    Insert

    Ranaspumin-2
    

    Tags

    His and iLOV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-GFP-CPDSalI

    Plasmid

    #38257

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    eGFP
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pEGFPN3 (Clonetech)

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pET30 SUMO-1 G97

    Plasmid

    #13381

    Depositor

    Mary Dasso

    Article

    Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.

    Insert

    SUMO-1, G97 (SUMO1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G97 mutant: lacks 4 amino acids from the C-termin…

    Available Since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

19. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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