We narrowed to 10,414 results for: 158

1. psiCHECK-1-miR-455-WT

   Plasmid

   #215881

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK-1-miR-376a1(3p)-I-type

   Plasmid

   #215883

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiCHECK-1-miR-589(5p)-I-type

   Plasmid

   #215884

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiCHECK-1-miR-589(3p)-I-type

   Plasmid

   #215885

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Tc1-9_Xt

   Plasmid

   #217158

   Purpose

   Mammalian expression vector for expression of Tc1-9_Xt transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   Tc1-9_Xt transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-AN-HA_aa 321-843 REV1

   Plasmid

   #201587

   Purpose

   Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA repair protein REV1 (REV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 321-843 of DNA repair prote…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgVRK1-hygro

   Plasmid

   #199640

   Purpose

   sgRNA guide against VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0319

   Plasmid

   #141581

   Purpose

   Lentiviral vector for overexpressing the MSLN transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSLN (MSLN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST_DR_1172_48-117 (pBS0288)

   Plasmid

   #185158

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DR_1172_48-117. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DR_1172_48-117
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221 NLRP1 delta787-843

    Plasmid

    #166821

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    _787-843

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 Ub-NLRP1 S1213 P1214R

    Plasmid

    #166830

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    T755N

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    R726W

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-D4 miR30-PIWIL2 ts2

    Plasmid

    #115870

    Purpose

    PIWIL2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    PIWIL2 shRNA (PIWIL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN161

    Plasmid

    #91580

    Purpose

    Express sgRNA targeting human CACNB2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CACNB2 (CACNB2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN014

    Plasmid

    #91588

    Purpose

    Express sgRNA targeting human CSMD1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CSMD1 (CSMD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN082

    Plasmid

    #91589

    Purpose

    Express sgRNA targeting human CUL3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CUL3 (CUL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL-PIAL2M-wt

    Plasmid

    #67909

    Purpose

    Inducible expression of PIAL2 in E.coli

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496

    Promoter

    P-lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAN1646

    Plasmid

    #127205

    Purpose

    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. p27-mVenus reporter (PB)

    Plasmid

    #190731

    Purpose

    The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    mVenus
    p27
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    p27-F62A-F64A

    Promoter

    EF1α

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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