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pTrc99S-ssDsbA-PD-4xDQNAT

Plasmid

#128399

Purpose

Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

H. influenzae protein D

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PorA-4xDQNAT

Plasmid

#128400

Purpose

Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

N. meningitidis PorA

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-139

Plasmid

#26329

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-139 (Mir139 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1224

Plasmid

#26337

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1224 (Mir1224 Mouse)

Expression

Mammalian

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only
pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGX

Plasmid

#62666

Purpose

Donor vector for Dre knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

Dre

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-217

Plasmid

#26333

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-217 (Mir217 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-1197

Plasmid

#26339

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1197 (Mir1197 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-216a

Plasmid

#26332

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-216a (Mir216a Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
RCAS (B) Eya2 (CT#665)

Plasmid

#13877

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Eya2 (EYA2 Chicken)

Use

Retroviral; Avian expression

Mutation

*

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1964

Plasmid

#26343

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1964 (Mir1964 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-224

Plasmid

#26335

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-224 (Mir224 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-888

Plasmid

#26316

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-888 (MIR888 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Pur-BFP

Plasmid

#248290

Purpose

Blue Fluorescent Protein expressing plasmid with Puromycin selection marker

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(Y86C)-GFP

Plasmid

#246840

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Tyrosine 86 to Cysteine

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(G75Q)-GFP

Plasmid

#246837

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with G75Q mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed G 75 to Q

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-R-GFP

Plasmid

#246831

Purpose

Express Arl13B-GFP in Arl13B KO RPE1 cells (sg1#a), resistance to sgRNA

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b-R-GFP (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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