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Plasmid#231722PurposeProtein expression in insect cellsDepositorInsertPOLR2A (POLR2A Human)
UseTagsFlag-3CExpressionInsectMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
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Tensin-2 Y483E
Plasmid#201794PurposeExpresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483EPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483F
Plasmid#201795PurposeExpresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483FPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483A
Plasmid#201796PurposeExpresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483APromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-K435L-L438K-peGFP-N1
Plasmid#228587PurposeExpresses OSCA1.2-K435L-L438K-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
UseTagsExpressionMammalianMutationK435L, L438KPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5e-Rubicon RH
Plasmid#216706PurposeBacterial protein expression of Rubicon RH domainDepositorInsertRubicon (RUBCN Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-TBK1
Plasmid#216709PurposeMammalian expression of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsTSFExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1616A/S1691A
Plasmid#215909PurposeExpression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D
Plasmid#215912PurposeExpression of full-length TNRC6A with S1332D/S1346D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332D, S1346DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332A/S1346A
Plasmid#215913PurposeExpression of full-length TNRC6A with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D
Plasmid#215914PurposeExpression of full-length TNRC6A with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616D, S1691DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A
Plasmid#215915PurposeExpression of full-length TNRC6A with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only