We narrowed to 15,020 results for: invs

1. pINSPECT_SigP:NLuc

   Plasmid

   #197263

   Purpose

   Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV.CBA.YFP.miR-E_shIrak1-2

   Plasmid

   #180398

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-2 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV.CBA.YFP.miR-E_shIrak1-3

   Plasmid

   #180399

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-3 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV.CBA.YFP.miR-E_shIrak1-1

   Plasmid

   #180397

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-1 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-VRN1-R249E-R289E-R296E

   Plasmid

   #159532

   Purpose

   Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

   Depositor

   Joshua Mylne

   Article

   King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

   Insert

   VERNALIZATION1 (VRN1 Mustard Weed)
   

   Use

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Three charge reversal mutations, namely R249E R28…

   Promoter

   T5

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-Nr4a1sgRNA2

   Plasmid

   #160946

   Purpose

   Guide RNA 2 to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 gRNA 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX304-ARF5-T161A-mNeonGreen

   Plasmid

   #162026

   Purpose

   Fast cycling ARF mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF5 (ARF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T161A

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR_P4-P1R:PER36modp

   Plasmid

   #128431

   Purpose

   Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

   Depositor

   George Haughn

   Article

   McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

   Insert

   PEROXISADE36 (AT3G50990 Mustard Weed)
   

   Use

   Gateway promoter entry clone

   Tags

   Expression

   Mutation

   Premature stop codons introduced into the Xero1 (…

   Promoter

   PEROXISADE36

   Available since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCDF1-MCS2-EF1-Puro-RGS7-E383K

   Plasmid

   #113731

   Purpose

   Express mutant RGS7- E383K- in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E383K

   Promoter

   CMV

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF1-MCS2-EF1-Puro-RGS7-R44C

    Plasmid

    #113729

    Purpose

    Express mutant RGS7- R44C- in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP Kind2 S159/181/666E

    Plasmid

    #105314

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCh-646

    Plasmid

    #92289

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 CED 7W7A (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215 with the following point …

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-PIAL2M-sim1

    Plasmid

    #67908

    Purpose

    Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496; VEDL 425-428 AAA

    Promoter

    P-lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mCardinal-Keratin-17

    Plasmid

    #56164

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pCMV6-Entry IL36RN R48W

    Plasmid

    #58324

    Purpose

    entry vector for human IL36RN R48W mutant

    Depositor

    Francesca Capon

    Article

    Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

    Insert

    IL36RN (IL36RN Human)
    

    Use

    Entry vector

    Tags

    myc-DDK

    Expression

    Mutation

    R48W: c.142C>T, p.Arg48Trp

    Promoter

    CMV

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCMV6-Entry IL36RN P76L

    Plasmid

    #58322

    Purpose

    entry vector for human IL36RN P76L mutant

    Depositor

    Francesca Capon

    Article

    Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

    Insert

    IL36RN (IL36RN Human)
    

    Use

    Entry vector

    Tags

    myc-DDK

    Expression

    Mutation

    P76L: c.227C>T,p.Pro76Leu​

    Promoter

    CMV

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV6-Entry IL36RN Κ35R

    Plasmid

    #58320

    Purpose

    entry vector for human IL36RN K35R mutant

    Depositor

    Francesca Capon

    Article

    Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

    Insert

    IL36RN (IL36RN Human)
    

    Use

    Entry vector

    Tags

    myc-DDK

    Expression

    Mutation

    Κ35R: c.104A>G, p.Lys35Arg​

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid

    #50914

    Purpose

    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C N-term deletion of aa13-222 in hNKCC1…

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

    Plasmid

    #50910

    Purpose

    Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid

    #50865

    Purpose

    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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