We narrowed to 59,421 results for: cloning

1. pDest-676 (attR4-attR3)

   Plasmid

   #161888

   Purpose

   Multisite Gateway (attR4-attR3) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hs.HRAS G12V

   Plasmid

   #83184

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12V mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12V

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV PIG (Puro IRES GFP empty vector)

   Plasmid

   #21654

   Purpose

   Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETMCSI-T4P_Y320A

   Plasmid

   #122522

   Purpose

   Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

   Depositor

   Gottfried Otting

   Article

   Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

   Insert

   gp43 - DNA polymerase (43 Escherichia virus T4)
   

   Expression

   Bacterial

   Mutation

   tyrosine 320 changed to alanine

   Promoter

   T7 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PresentER-Cassette GFP

   Plasmid

   #102942

   Purpose

   PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGF007

   Plasmid

   #48847

   Purpose

   Provides a kanamycin resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   pNOS::KanamycinR::tNOS
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREXC3GH

   Plasmid

   #47077

   Purpose

   FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Lactococcus lactis expression

   Tags

   3C protease site, GFP, and His

   Promoter

   NIS (nisin)

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJW1310

    Plasmid

    #61253

    Purpose

    Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    U6 promoter
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC27

    Plasmid

    #117478

    Purpose

    mobilizable, SRB-stable, kanamycin-resistant cloning vector

    Depositor

    Judy Wall

    Article

    Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. DDB1

    Plasmid

    #25246

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DDB1_1 (DDB1 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME Integrin beta 1b

    Plasmid

    #105976

    Purpose

    entry clone for gateway cloning, Full length Integrin beta 1b without stop

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXCA3H

    Plasmid

    #47072

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXNH3CA

    Plasmid

    #47069

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), AVI-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hs.HRAS C186S

    Plasmid

    #83189

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), C186S mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    C186S

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGB003

    Plasmid

    #48821

    Purpose

    Provides a default dummy as GreenGate N-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    B-Dummy
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. 599 p3E-mcs1

    Plasmid

    #49004

    Purpose

    Multiple cloning site in 3' gateway entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Gateway 3' entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCZGY2849

    Plasmid

    #74112

    Purpose

    Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    miniSOG(Q103L)
    

    Use

    Gateway topo cloning

    Tags

    PH

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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