We narrowed to 13,608 results for: crispr cas9

1. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

   Plasmid

   #242681

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

   Plasmid

   #242672

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

   Plasmid

   #242674

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

   Plasmid

   #242680

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. modRNAc1-VP64dCas9VP64-2A-MPH

   Plasmid

   #216388

   Purpose

   this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   VP64dCas9VP64-2A-MPH
   

   Use

   CRISPR

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-HiFi Cas9

   Plasmid

   #140563

   Purpose

   Expresses HiFi Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   R691A

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. modRNAc1-Cas9-2A-p53DD

   Plasmid

   #216389

   Purpose

   this vector can be used to make modRNA expressing Cas9 and p53DD

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   Cas9-2A-p53DD
   

   Use

   CRISPR

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 spCas9-mSA

   Plasmid

   #113096

   Purpose

   pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   Synthetic Biology

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. modRNAc1-Cas9-2A-puro

   Plasmid

   #216390

   Purpose

   this vector can be used to make modRNA expressing Cas9 and puro resistance gene

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   Cas9-2A-puro
   

   Use

   CRISPR

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-nCas9n-A71

    Plasmid

    #125644

    Purpose

    micro-injection of poly-adenylated nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    nCas9n
    

    Use

    Rna in vitro transcription vector

    Promoter

    T3

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-GFP-VPR

    Plasmid

    #181907

    Purpose

    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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