We narrowed to 4,411 results for: human cdnas

1. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

   Plasmid

   #205604

   Purpose

   Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinΔGTP-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A, N229A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

   Plasmid

   #115788

   Purpose

   Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   LRP5 (LRP5 Human)
   

   Use

   Tags

   3xflag

   Expression

   Mammalian

   Mutation

   contains silent mutation that creates an internal…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-eGFP-PC1-3HA

   Plasmid

   #108406

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   3HA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-hPC1_4106-eGFP-3HA

   Plasmid

   #108399

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-PHLPP1 N-terminal truncation

   Plasmid

   #22404

   Purpose

   Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-MAVS-M1A500(KaganF60)

   Plasmid

   #52003

   Purpose

   Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M1A-500
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala and deleted the C-terminal t…

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3.1-5'dGFP3'

   Plasmid

   #26425

   Depositor

   Erin Schuman

   Article

   Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

   Insert

   5'dGFP3'
   

   Use

   Tags

   CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pY016 (pcDNA3.1-hLbCpf1)

   Plasmid

   #69988

   Purpose

   Expresses humanized LbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Flag and Ha

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY004 (pcDNA3.1-hFnCpf1)

    Plasmid

    #69976

    Purpose

    Expresses humanized FnCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hFnCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 NQO1-Flag

    Plasmid

    #61729

    Purpose

    Expresses human NQO1 Flag tagged at C-terminus

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-UBXD3-Strep-HA

    Plasmid

    #113488

    Purpose

    Expression of human UBXD3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD3 (UBXN10 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid

    #59313

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele, Tito Serafini

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY014 (pcDNA3.1-hMbCpf1)

    Plasmid

    #69986

    Purpose

    Expresses humanized MbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hMbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid

    #59314

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Peter Scheiffele, Tito Serafini

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/TO-p37-Strep-HA

    Plasmid

    #113485

    Purpose

    Expression of human p37 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p37 (UBXN2B Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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