We narrowed to 6,526 results for: poly

1. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

   Plasmid

   #52093

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

   Plasmid

   #52092

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-PARP1-Apex2–eGFP

   Plasmid

   #211555

   Purpose

   A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   Apex2-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-PARP1-E988K

   Plasmid

   #211580

   Purpose

   CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains E988K mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-PARP1–eGFP

   Plasmid

   #211552

   Purpose

   A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. MG1655 tnaA-

   Bacterial Strain

   #228513

   Purpose

   Indole-responsive Phage Infection Assay using LARP and T7 Phage with gp1 knock-out

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Bacterial Resistance

   None

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

9. US0 pyrF-/hisB-/tnaA-

   Bacterial Strain

   #228512

   Purpose

   Indole-responsive Growth Selection

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Bacterial Resistance

   Tetracycline

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

10. pSLfa-PUb-MCS

    Plasmid

    #52908

    Purpose

    Aedes aegypti polyubiquitin promoter followed by MCS

    Depositor

    Zach Adelman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae aegptyi polyubiquitin

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOpen-T4gene43

    Plasmid

    #165524

    Purpose

    Polymerase that replicates viral genomic DNA.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pOpen-Pwopol

    Plasmid

    #165562

    Purpose

    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Pwo DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pOpen-ECOpolA

    Plasmid

    #165532

    Purpose

    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    E. coli DNA Polymerase I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. DH10beta F' DOT sbcC (donor strain)

    Bacterial Strain

    #12082

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Bacterial Resistance

    Tetracycline

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOpen-KTHEAQpol

    Plasmid

    #165542

    Purpose

    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    KlenTaq1 polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pOpen-Tlipol (exo-)

    Plasmid

    #165523

    Purpose

    DNA polymerase; lacks 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Tli DNA polymerase (exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pOpen-9N7polA

    Plasmid

    #165500

    Purpose

    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    9 degrees N-7 DNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge