We narrowed to 31,788 results for: son
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Plasmid#120444PurposeBarcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pDHt/sk-PE
Plasmid#92126PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reeseiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPPromoterPpdcAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorPublicationInsertTT-ISceI-dfrA
UseTemplateAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-LNS2
Plasmid#220083PurposeHigh affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPITPNM3 (PITPNM3 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro
Plasmid#183410PurposepiggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKRAB-dCas9-ecDHFR
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterTRE3GAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-FKBP-PTEN
Plasmid#116866PurposeRecruitable PTEN dual-specificity phosphataseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPTEN (PTEN Human)
TagsFKBP12 and mCherryExpressionMammalianAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-EFS-HypaSpCas9-P2A-puro
Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Occludin-N-10
Plasmid#54212PurposeLocalization: Tight Junctions, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOccludin (OCLN Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorAvailable sinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PDHA1-N-10
Plasmid#58195PurposeLocalization: Mitochondria, Excitation: N/A, Emission: N/ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDHA1 (PDHA1 Human)
TagsSNAP tagExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgLAP3
Plasmid#19704DepositorInsertpgLAP3
TagsEGFP, S peptide, and TEVExpressionMammalianAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mito-mGreenLantern
Plasmid#164461PurposeLocalization of mGreenLantern fluorescent protein to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
TagsHuman COX8a first 29 amino acidsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags6xHis tag, (-30)dGFP, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_003
Plasmid#192505PurposeFor cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFPDepositorInserthU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
UseLentiviralPromoterhU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
TagsHA, HisExpressionMammalianPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only