We narrowed to 25,308 results for: Nes
-
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]
Plasmid#240410PurposeFor Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)DepositorInsertsExpressionInsectPromoter3xP3 and Fibroin-L proximal promoter (Plodia inte…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBa.M6PR-GFP
Plasmid#224407PurposeMammalian expression of M6PRDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215327PurposeLentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertMUC5AC Promoter
UseLentiviral and LuciferaseExpressionMammalianPromoterMUC5ACAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-CRM197-4xDQNAT
Plasmid#128403PurposePeriplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertDiphtheria toxin
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating G52E mutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF5B
Plasmid#134625PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.MARK2-Halo
Plasmid#224414PurposeMammalian expression of MARK2DepositorAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215328PurposeLentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertFOXJ1 Promoter
UseLentiviral and LuciferaseExpressionMammalianPromoterFOXJ1Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
TagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only