We narrowed to 25,030 results for: Nes

1. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBV-luc TOP2A 1kb promoter

   Plasmid

   #138274

   Purpose

   Luciferase reporter plasmid driven by TOP2A promoter

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A promoter sequence
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-ING1a

   Plasmid

   #79069

   Purpose

   Expression of p47ING1 in mammalian cells

   Depositor

   Karl Riabowol

   Article

   Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

   Insert

   ING1 (p47 ING1A isoform) (ING1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pB.FRB-3myc-KLC1a

   Plasmid

   #134627

   Purpose

   Mammalian expression of mouse KLC1a

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KLC1a (Klc1 Mouse)
   

   Use

   Tags

   FRB, 3myc

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ps226

   Plasmid

   #133557

   Purpose

   RNAi construct for knock-down of CDKN1A (Cip1, p21)

   Depositor

   David Livingston

   Article

   Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE1059

   Plasmid

   #186628

   Purpose

   Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

   Inserts

   scbA
   scbB
   scbC
   

   Use

   Synthetic Biology

   Tags

   6xHis and 6xHis-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   Synthetic promoter B10, Synthetic promoter B19, a…

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP71-mtGFP-llo-lama4-alg8

   Plasmid

   #174605

   Purpose

   expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2/V5 hsa-mir-455

   Plasmid

   #26319

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-455 (MIR455 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXB7 (human) HIS-tag pET

    Plasmid

    #8538

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.2/V5 hsa-mir-499

    Plasmid

    #26320

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-499 (MIR499A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2/V5 mmu-mir-208a

    Plasmid

    #26331

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-208a (Mir208a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

16. L309-myc-seipin-CT

    Plasmid

    #64063

    Purpose

    myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Contains Arg326 to Ser443

    Promoter

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. HOXB5 (human) pBluescript

    Plasmid

    #8531

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    makes protein in vitro transcription/translation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1‐Afu rnhB

    Plasmid

    #108694

    Purpose

    For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Insert

    rnhB (AF_RS03165 Archaeoglobus fulgidus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG415-ADH1p-HA-rap1-K9R

    Plasmid

    #84531

    Purpose

    This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    ADH1

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pAG415-GPDp-EGFP-rap1-K3NR

    Plasmid

    #84533

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,117,118)R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only