We narrowed to 9,260 results for: Pol

1. env8 7U J1/13

   Plasmid

   #99829

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 7U J1/13
   

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 converted to 7 U's

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. env8 AC rep J1/13

   Plasmid

   #99830

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 AC rep J1/13
   

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 converted to repeating AC's

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. env8 25nt J1/13

   Plasmid

   #99836

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 25nt J1/13
   

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 with 25 nucleotides

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-HexHis

   Plasmid

   #72983

   Purpose

   Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Polyhistidine (HHHHHH) N-terminal tag
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 RRN3

   Plasmid

   #67112

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RRN3 (RRN3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. CS2-GFP-nopo

   Plasmid

   #47964

   Purpose

   Expression of Fly nopo

   Depositor

   Laura Lee

   Article

   Merkle et al Development. 2009 Feb;136(3):449-59. doi

   Insert

   no poles (nopo Fly)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Apex2-Venus-CAAX

   Plasmid

   #168511

   Purpose

   Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

   Insert

   Apex2
   

   Tags

   Venus-CAAX

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG vGlut1 mCherry

    Plasmid

    #105010

    Purpose

    Excitatory presynaptic marker

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScPPX2

    Plasmid

    #112877

    Purpose

    Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

    Depositor

    Ursula Jakob, Michael Gray

    Article

    Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Tags

    6X His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGE013

    Plasmid

    #108681

    Purpose

    All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB6364

    Plasmid

    #106151

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAtetO_6xHis-Klentaq

    Plasmid

    #157862

    Purpose

    Plasmid for the aTc-inducible expression of the Klentaq

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    Klentaq
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    tet PA

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG mito-mEos2

    Plasmid

    #168515

    Purpose

    Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

    Insert

    mito-mEos2
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    GUS

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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