We narrowed to 162,449 results for: addgene

1. pHR-IDR6-mCh-Cry2WT-NLS

   Plasmid

   #221921

   Purpose

   Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR6 (aa 1856-2090) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR6 region (aa 1856-2090); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-IDR1-mCh-Cry2WT-NLS

   Plasmid

   #221918

   Purpose

   Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR1 (aa 72-175) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR1 region (aa 72 - 175); inser…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #219653

   Purpose

   General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

   Depositor

   Thomas Oertner

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

   Expression

   Mammalian

   Mutation

   Promoter

   nEf Addgene # 149296

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTB3-FLAG tag

   Plasmid

   #210204

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   FLAG-tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTB3-AviD tag

   Plasmid

   #210203

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   AviD-tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA5_8xCTS-ECFP-pA

   Plasmid

   #200247

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 5

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA5_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA3_8xCTS-ECFP-pA

   Plasmid

   #200245

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 3

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA3_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgRNA2_8xCTS-ECFP-pA

   Plasmid

   #200244

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 2

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA2_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgRNA1 _8xCTS-ECFP-pA

   Plasmid

   #200243

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 1

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA1_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA4 _8xCTS-ECFP-pA

    Plasmid

    #200246

    Purpose

    Mammalian transfections; 8xCTS-ECFP reporter construct 4

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA4_8xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK BirA* zeo

    Plasmid

    #192332

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-BirA

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_TT BirA* w ccdB

    Plasmid

    #192310

    Purpose

    Gateway entry vector for cloning

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT 3xFLAG-Linker

    Plasmid

    #192305

    Purpose

    Gateway entry vector for cloning

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    Linker
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Venus-Bik-L61G-pEGFP-C1

    Plasmid

    #177426

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Leucine 61 replaced with glycine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid

    #177428

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycine 154 replaced with STOP codon

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. NODAL-T7TS

    Plasmid

    #115250

    Purpose

    For in vitro transcription of human NODAL open reading frame RNA

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NODALvar-T7TS

    Plasmid

    #115251

    Purpose

    For in vitro transcription of a human NODAL splice variant open reading frame RNA

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL splice variant (NODAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. H2B-MiniE-mEm

    Plasmid

    #181991

    Purpose

    MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    H2B-MiniE-mEm
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6DXH (USP05)

    Plasmid

    #119968

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    USP05 (USP5 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 171-290

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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