We narrowed to 159,868 results for: addgene

1. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. miReporter-PGK

   Plasmid

   #82477

   Purpose

   microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GalToxBFP

   Plasmid

   #68073

   Purpose

   mammalian expression of GC localized oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   GalT signal anchor transmembrane domain
   

   Use

   Tags

   moxBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRE

   Plasmid

   #26745

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   iCre expression cassette
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHPRT-DRGFP

   Plasmid

   #26476

   Purpose

   DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

   Insert

   pHPRT-DRGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIG-Aalpha WT

   Plasmid

   #10884

   Depositor

   William Hahn

   Article

   Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

   Insert

   PP2A Aalpha (PPP2R1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCrlM

   Plasmid

   #122444

   Purpose

   Expresses myc tagged mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER-moxMaple3

    Plasmid

    #120885

    Purpose

    mammalian expression of ER localized moxMaple3

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Insert

    moxMaple3
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. LC3B sgRNA

    Plasmid

    #207556

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CACTGAATACCAGCGCCTAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LacZ_pHIVEGFP

    Plasmid

    #191251

    Purpose

    Expresses LacZ in mammalian cells (used as a control)

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

    Insert

    LacZ
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ptet

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hIRE1a wt

    Plasmid

    #20744

    Depositor

    Fumihiko Urano

    Article

    Lipson et al PLoS ONE. 2008 . 3(2):e1648.

    Insert

    IRE1 (ERN1 Human)
    

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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