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We narrowed to 3,052 results for: erf

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Showing: 2101 - 2120 of 3052 results

  2. pRC/CMV-TYK21054-5FF-VSV

    Plasmid
    #139347
    Purpose
    Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop
    Depositor
    Sandra Pellegrini
    Publication
    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Expression
    Mammalian
    Mutation
    V362F-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    139347-plasmid-map-sequence-id-291878

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  3. pRC/CMV-TYK2K930R-FF-VSV

    Plasmid
    #139348
    Purpose
    Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop
    Depositor
    Sandra Pellegrini
    Publication
    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Tags
    VSV
    Expression
    Mammalian
    Mutation
    V362F-K930R-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    139348-plasmid-map-sequence-id-275739

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  4. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Publication
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pEF2-FL-hMer_gR1

    Plasmid
    #225993
    Purpose
    Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor
    Depositor
    Raymond Birge, Sergei Kotenko
    Publication
    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
    Expression
    Mammalian
    Promoter
    EF1-a
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225993-plasmid-map-sequence-id-454327

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  7. mEGFP-N1 YAPS127A- L308/311/325/318E (4LE)

    Plasmid
    #166466
    Purpose
    Expresses fusion of mEGFP and YAPS127A- L308/311/325/318E (4LE)
    Depositor
    Shuguo Sun
    Publication
    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEGFP, YAPS127A- L308/311/325/318E (4LE) (YAP1 Human)
    Expression
    Mammalian
    Mutation
    YAPS127A- L308/311/325/318E (4LE)
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166466-plasmid-map-sequence-id-330045

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  8. pcDNA3-sACE2-T92Q-sfGFP

    Plasmid
    #145178
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145178-plasmid-map-sequence-id-292625

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  11. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Staphylococcus aureus, Human)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  12. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Staphylococcus aureus, Human)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  16. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (CYP8B1 Staphylococcus aureus, Human)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  17. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid
    #185448
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Publication
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    See Depositor Comments Below
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185448-plasmid-map-sequence-id-364976

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  18. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192785-plasmid-map-sequence-id-379661

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  19. 6-ISG-IFN

    Plasmid
    #231488
    Purpose
    Standard for type I interferon signature (6 genes)
    Depositor
    Stefano Volpi
    Publication
    Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d
    Inserts
    TBP (TBP Human)
    IFI27 (IFI27 Human)
    IFI44L (IFI44L Human)
    IFIT1 (IFIT1 Human)
    ISG15 (ISG15 Human)
    RSAD2 (RSAD2 Human)
    SIGLEC1 (SIGLEC1 Human)
    HPRT1 (HPRT1 Human)
    18s (RPS18 Human)
    G6PD (G6PD Human)
    Use
    Qpcr plasmid standard
    Mutation
    partial sequence
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231488-plasmid-map-sequence-id-469002

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  20. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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Showing: 2101 - 2120 of 3052 results