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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP30-CFP

Plasmid

#178304

Purpose

CoTrIP plasmid with HSP30 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP30pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP30 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP26-CFP

Plasmid

#178306

Purpose

CoTrIP plasmid with HSP26 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP26pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP26 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Expression

Yeast

Promoter

GLC3 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

Plasmid

#178999

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pXT

Plasmid

#107574

Purpose

Contains the VGF promoter (about 700 bp) plus 800 bp of 5' untranslated sequence. The HindIII (5')-HincII(3') fragment acts as a NGF-inducible promoter

Depositor

Andrea Levi

Article

Possenti et al Proc Natl Acad Sci U S A. 1992 May 1;89(

Insert

VGF promoter (Vgf Rat)

Use

Cloning vector

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x5

Plasmid

#63605

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-2X snRP1

Plasmid

#99688

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

Plasmid

#99679

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64-p65(101-261)-RTA(125-190)

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pXFokI-dCas9

Plasmid

#60901

Purpose

Dual Expression Vector for FokI-dCas9 and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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