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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,041 results for: Ank

Showing: 5301 - 5350 of 7041 results

  1. pHBP1-donor

    Plasmid
    #112336
    Purpose
    CRISPR donor plasmid to tag human transcription factor HBP1 with GFP
    Depositor
    Kevin White
    Insert
    HBP1 homology arms flanking EGFP-IRES-Neo cassette (HBP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112336-plasmid-map-sequence-id-228570

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  2. pZNF785-donor

    Plasmid
    #112317
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP
    Depositor
    Kevin White
    Insert
    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112317-plasmid-map-sequence-id-228521

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  3. pZNF354B-donor

    Plasmid
    #112309
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP
    Depositor
    Kevin White
    Insert
    ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:5:178884427:G>A, hg38:5:178883445:C>T,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112309-plasmid-map-sequence-id-228461

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  6. psglessClonePX

    Plasmid
    #87193
    Purpose
    used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice
    Depositor
    Thomas Cech
    Article
    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87193-plasmid-map-sequence-id-170165

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  8. pCfB2229

    Plasmid
    #67537
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    67537-plasmid-map-sequence-id-116409

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  17. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  23. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  29. pCfB2188

    Plasmid
    #67296
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67296-plasmid-map-sequence-id-124518

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  33. pMT3928

    Plasmid
    #113331
    Purpose
    control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Article
    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:
    Inserts
    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113331-plasmid-map-sequence-id-218711

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  35. 2xMARS

    Plasmid
    #205233
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Use
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205233-plasmid-map-sequence-id-404744

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  40. pJAT77

    Plasmid
    #204295
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204295-plasmid-map-sequence-id-406178

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  42. pUC-hrDNA-12.0

    Plasmid
    #206320
    Purpose
    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)
    Depositor
    Brian McStay
    Article
    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1
    Insert
    Intergenic spacer of human rDNA repeat
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206320-plasmid-map-sequence-id-405354

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  46. pVP073

    Plasmid
    #222076
    Purpose
    eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222076-plasmid-map-sequence-id-440974

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  47. pVP074

    Plasmid
    #222077
    Purpose
    eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222077-plasmid-map-sequence-id-440969

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  48. pVP075

    Plasmid
    #222078
    Purpose
    AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222078-plasmid-map-sequence-id-440967

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Showing: 5301 - 5350 of 7041 results