We narrowed to 4,541 results for: Alk

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nSaCas9 M741 miniABEMax V82G

Plasmid

#135383

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M741 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 N120 miniABEMax V82G

Plasmid

#135384

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 N120 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 AIDx no UGI

Plasmid

#135349

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 APOBEC3 no UGI

Plasmid

#135350

Purpose

Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-rAPOBEC3-ID-1058

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 AIDx with UGI

Plasmid

#135354

Purpose

Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

hAIDx-nSpyCas9 aa-UGI

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 ABEMax

Plasmid

#135356

Purpose

Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-ABEMax aa 1058

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 3

Plasmid

#135359

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 CP nSpyCas9 ABEMax

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 V82G ABEMax

Plasmid

#135361

Purpose

Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1058 nSpyCas9 V82G ABEMax

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 V82G ABEMax Strategy 3

Plasmid

#135363

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 V82G ABEMax

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-202

Plasmid

#135344

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-202

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 NPm

Plasmid

#126593

Purpose

Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 1-65 and L329A/L331A in t…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 CCm

Plasmid

#126594

Purpose

Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 203-228 and siRNA resista…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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289aa Met133, 138, 186Ileu ELL2

Plasmid

#127272

Purpose

Expresses 289aa human ELL2 with Met133, 138, 186Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)

Expression

Mammalian

Mutation

Met133, 138, 186 Ileu, cut with XbaI blunt fillin

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-HSPE-sh1

Plasmid

#92033

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #1 to HPSE (Hpse Mouse)

Use

Retroviral

Available Since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSIREN-RetroQ-HSPE-sh2

Plasmid

#92034

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #2 to HPSE (Hpse Mouse)

Use

Retroviral

Available Since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

Plasmid

#62579

Purpose

Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)GαsL-260-NLuc

Plasmid

#207158

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(260)N

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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