We narrowed to 218,689 results for: CIL

1. pBT3019

   Plasmid

   #122541

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemOrange2::SKL
   

   Tags

   SKL

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IPU-FLAG-NEK9T210A

   Plasmid

   #168270

   Purpose

   Expresses kinase-dead NEK9 mutant in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 210 to alanine

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLNCX2-Orai1

   Plasmid

   #89818

   Purpose

   Express Orai1 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPSP6+t-gfp

   Plasmid

   #48130

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNAZeo(-)HSC73AS

   Plasmid

   #86031

   Purpose

   Antisense for pCDNAZeo(-)HSC73

   Depositor

   Janice Blum

   Article

   Zhou et al Immunity. 2005 May;22(5):571-81.

   Insert

   heat shock cognate protein 70 (hsc70) (HSPA8 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE-FDXR mut

   Plasmid

   #16611

   Depositor

   Bert Vogelstein

   Article

   Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

   Insert

   FDXR (FDXR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion mutant, contains aa 1-42

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. CoV2-N-D377Y

   Plasmid

   #177957

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D377Y) (N )
   

   Expression

   Mammalian

   Mutation

   D377Y

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST47-Arl13b17

   Plasmid

   #40875

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains Arl13b amino acids 1-199

   Promoter

   CMV

   Available Since

   March 13, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5FRT/TO-UBXD1-Strep-HA

   Plasmid

   #113476

   Purpose

   Expression of human UBXD1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD1 (UBXN6 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   C174T silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-agrBD-IV

    Plasmid

    #53440

    Purpose

    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus (with D29Y mutation in agrD)
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    D to Y mutation of residue 29 of the agrD gene (c…

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

    Plasmid

    #172103

    Purpose

    Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Bba_R0040 TetR

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IPU-GFP-NEK9W967A

    Plasmid

    #168271

    Purpose

    Expresses LIR (LC3-interacting region) mutant NEK9

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 979 to alanine

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Hyg-FHIT

    Plasmid

    #16410

    Depositor

    Bert Vogelstein

    Article

    Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

    Insert

    FHIT Hyg targeting (FHIT Human)
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

18. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Expression

    Mammalian

    Mutation

    P13L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Expression

    Mammalian

    Mutation

    S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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