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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,329 results for: pcDNA

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Showing: 2121 - 2140 of 12329 results

  3. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid
    #207151
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207151-plasmid-map-sequence-id-410283

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  4. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid
    #207152
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207152-plasmid-map-sequence-id-410279

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  6. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  7. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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  8. pcDNA3-HA2-PARC

    Plasmid
    #20937
    Depositor
    Yue Xiong
    Insert
    p53-associated parkin-like cytoplasmic protein (CUL9 Human)
    Tags
    HA2
    Expression
    Mammalian
    Mutation
    S1049N, C1076R, R2389L
    Available Since
    April 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    20937-plasmid-map-sequence-id-218362

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  15. pcDNA3.1-ccdB-3xFLAG

    Plasmid
    #214984
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214984-plasmid-map-sequence-id-440018

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  18. pcDNA3.1.Gαi2-LgB91

    Plasmid
    #134358
    Purpose
    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi2-LgB91 (GNAI2 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134358-plasmid-map-sequence-id-306397

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  19. pcDNA3-GFP-MMP7

    Plasmid
    #11989
    Depositor
    Steven Johnson
    Insert
    MMP7 (MMP7 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11989-plasmid-map-sequence-id-133484

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Showing: 2121 - 2140 of 12329 results