We narrowed to 11,729 results for: pcDNA

1. pcDNA Flag-RARalpha

   Plasmid

   #35555

   Depositor

   Harish Srinivas, Jonathan Kurie

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1_mGFP-FGFR3-K508M

   Plasmid

   #191753

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1523A>T (p.K508M) + silent mutation c.1515A&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-RAB-AKARev

   Plasmid

   #118478

   Purpose

   ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-AKARev
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 AKT3 HA (CT-gfp)

   Plasmid

   #27293

   Depositor

   Morris Birnbaum

   Insert

   protein kinase B gamma (Akt3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y473F

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3 Flag WDR5

   Plasmid

   #15552

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Wdr5 (Wdr5 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA5-Flag-BRD4-BD

   Plasmid

   #90005

   Purpose

   expresses BD mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 140N to 140A, 433N to 433A

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5FRT/TO-Strep/HA-UBE2J1

   Plasmid

   #124669

   Purpose

   Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-nuc-Nb80

   Plasmid

   #205519

   Purpose

   Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb80
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Flag-Smad4

    Plasmid

    #80888

    Purpose

    mammalian expression of Smad4

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-300(HAT-)

    Plasmid

    #23254

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H1415A E1423A Y1424A L1428S Y1430A H1434A

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-SypHluorin 2x (S2x)

    Plasmid

    #37004

    Depositor

    Stephen Heinemann, Yongling Zhu

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 2x (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Two copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-pri-let-7b

    Plasmid

    #51378

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    pri-let-7b (MIRLET7B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-YFP-PKD2

    Plasmid

    #31821

    Depositor

    Alexandra Newton

    Article

    Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

    Insert

    YFP-Protein Kinase D2 (PRKD2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-PalmPalm-ClopHensor

    Plasmid

    #25940

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    PalmPalm-ClopHensor
    

    Use

    Tags

    Gap20 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (20AA) of Gap-43

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid

    #191756

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1948A>C (p.K650Q) + silent mutation c.1938C&…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-BaLgp140-8his

    Plasmid

    #100924

    Purpose

    Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after D6…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-sACE2(WT)-sfGFP

    Plasmid

    #145171

    Purpose

    Mammalian expression plasmid for soluble ACE2-super folder GFP fusion

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge