We narrowed to 17,558 results for: puromycin

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T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

Plasmid

#193356

Purpose

VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_003

Plasmid

#192505

Purpose

For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-DTAG-NTERM-GFP

Plasmid

#185775

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PB-EF1a-Puro-V8.2

Pooled Library

#178943

Purpose

Barcoding library for the macsGESTALT inducible lineage recording system

Depositor

Christopher Lengner

Article

Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only
EFSp-GFP-YAP

Plasmid

#174168

Purpose

Lentiviral vector to constitutively express YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-CAG-Brainbow3.2-PGK-PuroTK

Plasmid

#159928

Purpose

Brainbow transgenesis

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Inserts

NFPnls
mOrange2
EGFP
mKate2

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 WT

Plasmid

#121486

Purpose

2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Medium

Plasmid

#157670

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Medium-FT

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV MCL1 Puro

Plasmid

#140746

Purpose

Expresses MCL1 in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

MCL1 (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL-UCOE-SFFV-TLR-MCV1

Plasmid

#117111

Purpose

Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

UCOE SFFV Traffic Light Reporter 2.0

Use

Lentiviral; Reporter

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgIRF3#5

Plasmid

#127642

Purpose

Knock-out of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEUI(+)

Plasmid

#111834

Purpose

For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

Depositor

Hyunju Ro

Article

Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

Insert

GAL4-minimal VP16-EcR (EcR Synthetic)

Expression

Mammalian

Promoter

CMV, SV40, 10XUAS

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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LLP185 pLVP-dCas9-DNMT3a V2

Plasmid

#100936

Purpose

dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-mPERK-FLAG-IP

Plasmid

#101793

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PERK

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Perk (Eif2ak3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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ZNF217_pLX307

Plasmid

#98384

Purpose

Lentiviral expression of ZNF217

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ZNF217 (ZNF217 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-USP7

Plasmid

#99393

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

USP7 (USP7 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/gene

Pooled Library

#83989

Purpose

Mouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only