We narrowed to 6,661 results for: sas

1. AAV_CMV_CCR6-Nanoluc-V5

   Plasmid

   #199672

   Purpose

   expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   CCR6 (CCR6 Human)
   

   Use

   AAV

   Tags

   NanoLuc and V5

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6/V5-DEST-RINT1

   Plasmid

   #40124

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   RINT1 (RINT1 Human)
   

   Use

   Lentiviral

   Tags

   V5 epitope

   Expression

   Mammalian

   Mutation

   L270M relative to NM_021930

   Promoter

   CMV

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. FcD265A-FLAG

   Plasmid

   #65420

   Purpose

   Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

   Depositor

   Dane Wittrup

   Article

   Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

   Insert

   FcD265A-FLAG
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D265A

   Promoter

   Modified CMV Promoter

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pENTR-myr-AKT-HA

   Plasmid

   #31790

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Entry vector

   Tags

   HA and Myr

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

   Plasmid

   #211817

   Purpose

   AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   N-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

   Plasmid

   #211818

   Purpose

   AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   C-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-SCN1B-COMP5AP-AviTag-9xHis

   Plasmid

   #157099

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SCN1B (SCN1B Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-NCR3LG1-COMP5AP-AviTag-9xHis

   Plasmid

   #157255

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NCR3LG1 (NCR3LG1 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD649-HAsp-SEMA6D-COMP5AP-AviTag-9xHis

   Plasmid

   #157498

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA6D (SEMA6D Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_HA.NWS.VSVg_NGFR

    Plasmid

    #158243

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.NWS.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-LILRA3-COMP5AP-AviTag-9xHis

    Plasmid

    #157410

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LILRA3 (LILRA3 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-BTNL8-COMP5AP-AviTag-9xHis

    Plasmid

    #157225

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    BTNL8 (BTNL8 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8-PHF19

    Plasmid

    #68864

    Purpose

    GW entry vector for PHF19

    Depositor

    Adrian Bracken

    Article

    Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Gateway entry vector

    Promoter

    none

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

    Plasmid

    #117405

    Purpose

    SpyCas9 sgRNA 1 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-UNC5C-COMP5AP-AviTag-9xHis

    Plasmid

    #157343

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    UNC5C (UNC5C Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTT3-SP-6xHis-PVR(FL)-FLAG

    Plasmid

    #157622

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PVR (PVR Human)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-miRFP-6xFLAG

    Plasmid

    #161614

    Purpose

    Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    miRFP-6xFLAG
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid

    #158245

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.FLAG.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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