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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4-mCherry

Plasmid

#80269

Purpose

mammalian expression plasmid for Plk4-mCherry

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pN3FS-CYC1

Plasmid

#85783

Purpose

N-ICE plasmid pH3FS-CYC1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-NatMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Mutation

*(see below)

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKvenus-Scribble

Plasmid

#58738

Purpose

Expresses Venus-tagged human Scribble protein

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

Scribble (SCRIB Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pN3-3xFlag-Control

Plasmid

#107717

Purpose

Control expression plasmid for 3xFLAG-tagged pN3-contructs

Depositor

Guntram Suske

Article

Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

Type

Empty backbone

Tags

3xFlag

Expression

Mammalian

Available Since

April 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-M13-TEV-C-P2A-TdTomato

Plasmid

#92389

Purpose

expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Tags

TdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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