We narrowed to 2,318 results for: PUC19

1. qTAG-N-Blast-miniTurbo-LMNB1

   Plasmid

   #207774

   Purpose

   Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. qTAG-N-Blast-V5-LMNB1

   Plasmid

   #207779

   Purpose

   Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. qTAG-C-Solo-mScarlet-H3C2

   Plasmid

   #207781

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. qTAG-C-mNeon-Blast-H3C2

   Plasmid

   #207782

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-N-Blast-mNeon-ACTB

   Plasmid

   #207751

   Purpose

   Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-N-Solo-mScarlet-ACTB

   Plasmid

   #207750

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-N-Solo-mNeon-ACTB

   Plasmid

   #207749

   Purpose

   Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-N-Blast-moxGFP-TUBA1B

   Plasmid

   #207766

   Purpose

   Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. IR76b-T2A-QF2

   Plasmid

   #162523

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GR3-T2A-QF2

    Plasmid

    #162521

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. IR8a-T2A-QF2

    Plasmid

    #162520

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #199450

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

    Plasmid

    #199451

    Purpose

    CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

    Plasmid

    #199452

    Purpose

    CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IR25a-T2A-QF2

    Plasmid

    #162522

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Donor_C_FH_VIR

    Plasmid

    #186660

    Purpose

    Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Donor_N_FH_Shu

    Plasmid

    #186655

    Purpose

    Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Donor_N_FH_Piwi

    Plasmid

    #186651

    Purpose

    Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Donor_C_FH_Nbr

    Plasmid

    #186662

    Purpose

    Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDonor_N_FH_GLE

    Plasmid

    #186657

    Purpose

    Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge