We narrowed to 72,445 results for: ang
-
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG4_PDZ_3
Plasmid#103953PurposeProtein expression and purification of PSD95 PDZ3 domainDepositorInsertDLG4 (DLG4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-TagRFP-OcsT
Plasmid#71270PurposeEntry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-ASAP2s
Plasmid#101275PurposeGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertASAP2s
UseAAVTagsExpressionMammalianMutationPromoterCAMKIIaAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3T911A
Plasmid#120254PurposeExpression of ATP3-6 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
UseTagsExpressionYeastMutationT911 changed to A; Isoleucine 304 changed to Aspa…PromoterATP3Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(C141S)-EGFP
Plasmid#89635PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG, 6*His, and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCCPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTAL_RFP
Plasmid#43859DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.2
Plasmid#111069PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del2 pET28a
Plasmid#122116PurposeExpression Brg1 amino acids 1-837 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTags6XHis and His-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVTagsExpressionMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAL_GFP
Plasmid#43858DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
5XUAS IVS CsChrimson tdtomato_tr
Plasmid#111545PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertCsChrimson_tdtomato_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p27 T187A Luc
Plasmid#23048DepositorInsertp27 (CDKN1B Human)
UseTagsLuciferaseExpressionMammalianMutationT187A: mutation at codon 187 changing from T (ACG…PromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Neurod1-Atoh7HA3
Plasmid#44438DepositorInsertAtoh7-3xHA knock-in (Atoh7 Mouse)
UseCre/Lox and Mouse TargetingTagsNeurod1 left arm, Neurod1 right arm, and loxC2-Re…ExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3-Rac1
Plasmid#92039PurposeExpresses mEos3-Rac1 fusion proteinDepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-caRac1
Plasmid#92041PurposeExpresses mEos3 fusion with caRac1 (Q61L)DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationQ61L (constitutively active)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-C/EBPbZIP (pGEX-bZIPa)
Plasmid#12435DepositorInsert68 a.a. fragment of C/EBP alpha (CEBPA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C72A
Plasmid#122477Purposeexpresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LIN7A_PDZ_1
Plasmid#103918PurposeProtein expression and purification of LIN7 PDZ domainDepositorInsertLIN7A (LIN7A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-c
Plasmid#53078Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_3
Plasmid#103917PurposeProtein expression and purification of DLG1 PDZ3 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(m40)-luc
Plasmid#12425DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationPU.1 binding site mutation (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T142Q
Plasmid#108109PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut
Plasmid#19061DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsExpressionMammalianMutationTryptophan 199 changed to Alanine,and Proline 202…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
HTRA3_PDZ_1
Plasmid#103914PurposeProtein expression and purification of HTRA3 PDZ domainDepositorInsertHTRA3 (HTRA3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala10
Plasmid#87135PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorUseTagsExpressionBacterialMutationD196A, L197RPromoterwaaHp from E. coli as part of operon with ald* an…Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L16A/L17A
Plasmid#13478DepositorInsertMKP-1L16A/L17A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Leucine 16 to Alanine; changed Leucine 1…PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_2
Plasmid#103916PurposeProtein expression and purification of DLG1 PDZ2 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-31-Reporter
Plasmid#71871PurposeMammalian expression vector for the analysis of miR-31 activityDepositorInsertReverse complementary sequence of miR-31
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
IFITM3-N21del-V93-TAG
Plasmid#122480Purposeexpresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationChanged Valine 93 to amber codon and deleted amin…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brm BDmut-Flag pShuttle
Plasmid#122108PurposeExpression of full length Brm containing point mutations in its bromodomain in mammalian cellsDepositorInsertBrm (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_1
Plasmid#103915PurposeProtein expression and purification of DLG1 PDZ1 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant
Plasmid#19000DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 199 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
3695 pGEX4T-3 MLL AD mut.E
Plasmid#8867DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationILPSDIMDFVL --> ILPADIMDFVLPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a F656A
Plasmid#53054PurposeExpresses alpha subunit of F656A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Phenylalanine 656 to AlaninePromoterSP6Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a Y652A
Plasmid#53053PurposeExpresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Tyrosine 652 to AlaninePromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCHKU34.1-2.2
Plasmid#115534PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertPKS
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HTRA2_PDZ_1
Plasmid#103913PurposeProtein expression and purification of HTRA2 PDZ domainDepositorInsertHTRA2 (HTRA2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C105A
Plasmid#122478Purposeexpresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-V93-TAG
Plasmid#122479Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Valine 93 to amber codonPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C71A
Plasmid#122476Purposeexpresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
3704 pGEX4T-3 MLL AD L2854A
Plasmid#8876DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationL2854A: ILPSDIMDFVL --> ILPSDIMDFVAPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only