We narrowed to 3,004 results for: PHI-1
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Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab4B
Plasmid#61801PurposeHuman Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorAvailable SinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
POC1468
Plasmid#213756PurposeBsaI-associated non-switchable methylaseDepositorInsertNon-switchable methylase M2.BsaI
TagsHis6-tagExpressionBacterialPromoterT7Available SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1467
Plasmid#213755PurposeBsaI-associated non-switchable methylaseDepositorInsertNon-switchable methylase M2.BsaI
TagsHis6-tagExpressionBacterialPromoterT7Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6
Plasmid#176652PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
ExpressionYeastAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6B
Plasmid#176666PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pCAGGS
Plasmid#206076Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b GFP
Plasmid#89893PurposeMammalian expression of Beta1b GFPDepositorInsertCacnb1 (Cacnb1 Rat)
TagsGFPExpressionMammalianMutationGFP sequence inserted just before the Stop codonPromoteradenovirus major late promoterAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b mCherry
Plasmid#89892PurposeMammalian expression of Beta1b mCherryDepositorInsertCacnb1 (Cacnb1 Rat)
TagsmCherryExpressionMammalianMutationmCherry sequence inserted just before the Stop co…Promoteradenovirus major late promoterAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 DomI + DomII pcDNA3
Plasmid#206094Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channelDepositorAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 DomI pMT2
Plasmid#206127Purposeexpression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channelDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Cav2.2 DomI and DomII
Plasmid#89891PurposeMammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2DepositorInsertCacna1b (CACNA1B )
ExpressionMammalianMutationStop codon after amino acid 1154 (this is a trunc…Promoteradenovirus major late promoterAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRPromoterMHCAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHSV-TK
Plasmid#165801PurposeMinimal viral terminator derived from Herpes Simplex 1 thymidine kinase geneDepositorInsertHSV-TK
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only