We narrowed to 5,560 results for: pcas

1. pCAG-GST-ATG13 (363-450)

   Plasmid

   #210849

   Purpose

   Mammalian expression of ATG13 (363-450) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-FIP200 (1-640)-R334D-TSF

   Plasmid

   #210841

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R334D

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-FIP200 (1-640)-R326D-TSF

   Plasmid

   #210840

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R326D

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-FIP200 (1-640)-V314D-TSF

   Plasmid

   #210839

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V314D

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-FIP200 (1-640)-V44D-TSF

   Plasmid

   #210843

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V44D

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PET-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207387

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207382

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-GST-ATG13 (363-460)

    Plasmid

    #203553

    Purpose

    Mammalian expression of GST tagged ATG13 (363-460)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid

    #202530

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SAMD8 (SAMD8 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-ATG13 (363-517)-MBP

    Plasmid

    #203561

    Purpose

    Mammalian expression of ATG13 (363-517) with MBP tag

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    MBP-10His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-GST-ATG13 (460-517)

    Plasmid

    #203552

    Purpose

    Mammalian expression of GST tagged ATG13 (460-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-GST-ATG13 (450-517)

    Plasmid

    #203558

    Purpose

    Mammalian expression of GST tagged ATG13 (450-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-TSF-ATG13 (363-517)

    Plasmid

    #203550

    Purpose

    Mammalian expression of TwinStrep-Flag tagged ATG13 (363-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-GST-ATG13 (363-398)

    Plasmid

    #203554

    Purpose

    Mammalian expression of GST tagged ATG13 (363-398)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-GST-ATG13 (402-450)

    Plasmid

    #203557

    Purpose

    Mammalian expression of GST tagged ATG13 (402-450)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-CHMP2A-myc, L216D/L219D

    Plasmid

    #180645

    Purpose

    Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L216D, L219D

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 RPS6KL1 MIT, residues 20-140

    Plasmid

    #180623

    Purpose

    Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 20-140

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge