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pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin

Plasmid

#221861

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG Ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin

Plasmid

#221869

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polb(WT)-C-Hygro

Plasmid

#216777

Purpose

Flag-Polb(WT), C-terminal domain (AA 91-335) & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335)

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polb(TM)-C-Hygro

Plasmid

#216778

Purpose

Flag-Polb(TM), C-terminal domain (AA 91-335) & a hygromycin resistance cassette; TM = L301R/V303R/V306R

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335); TM = L301…

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polb(TM/DM)-C-Hygro

Plasmid

#216780

Purpose

Flag-Polb(TM/DM), C-terminal domain (AA 91-335) & a hygromycin resistance cassette; TM/DM = L301R/V303R/V306R/K206A/K244A

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335); TM/DM = L…

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207_∆polar_-3xFLAG

Plasmid

#231934

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆polar_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

∆polar

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-Nterm

Plasmid

#242942

Purpose

Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Nterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-C-term

Plasmid

#242943

Purpose

Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Cterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-foxA-C-term

Plasmid

#242944

Purpose

Encodes a C-terminal fragment of Spol-foxA to generate dsRNA for RNAi experiments in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-foxA

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Sc FLAG-Pol2-pRS425/GAL

Plasmid

#241978

Purpose

Over-express Sc FLAG-Pol2 (Pol epsilon large subunit) in yeast (episomal)

Depositor

Michael O'Donnell

Insert

FLAG-Pol2 (POL2 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Sc Pol1-FLAG-pRS402/GAL-L

Plasmid

#241975

Purpose

Over-express Sc Pol1-FLAG (Sc pol alpha - large subunit) in yeast (integrated)

Depositor

Michael O'Donnell

Insert

Pol1-FLAG (POL1 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Sc FLAG-POL4-pRS404/GAL-L

Plasmid

#241261

Purpose

Over-express Sc FLAG-POL4 in yeast (integrated)

Depositor

Michael O'Donnell

Insert

FLAG-POL4 (POL4 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Sc Pol3-FLAG(Ec)-pRSF405/GAL

Plasmid

#239198

Purpose

Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

Depositor

Michael O'Donnell

Insert

Pol3 (POL3 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Mutation

E. coli codon bias (synthetic gene), but same ami…

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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1162BX-CMV-EGFP-bGHpolyA

Plasmid

#235171

Purpose

Expresses EGFP

Depositor

Omar Akbari

Insert

EGFP

Expression

Mammalian

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-3xFLAG-PolyS

Plasmid

#181728

Purpose

Galactose inducible expression of 3xFLAG-PolyS

Depositor

Alejandro Chavez

Insert

3xFLAG-PolyS

Tags

3xFLAG

Expression

Yeast

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-3xFLAG-PolyA

Plasmid

#181726

Purpose

Galactose inducible expression of 3xFLAG-PolyA

Depositor

Alejandro Chavez

Insert

3xFLAG-PolyA

Tags

3xFLAG

Expression

Yeast

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-3xFLAG-PolyL

Plasmid

#181727

Purpose

Galactose inducible expression of 3xFLAG-PolyL

Depositor

Alejandro Chavez

Insert

3xFLAG-PolyL

Tags

3xFLAG

Expression

Yeast

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-3xFLAG-PolyC

Plasmid

#181729

Purpose

Galactose inducible expression of 3xFLAG-PolyC

Depositor

Alejandro Chavez

Insert

3xFLAG-PolyC

Tags

3xFLAG

Expression

Yeast

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226768

Purpose

vector with mScarlet fluorescent protein and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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3'dmDR-CMV-CasRx-bGHpolyA

Plasmid

#229556

Purpose

Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

Depositor

Omar Akbari

Insert

CasRx

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203A-CMV-5’DR-EGFP-bGHpolyA

Plasmid

#221452

Purpose

EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end

Depositor

Omar Akbari

Insert

eGFP

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203B-CMV-3’DR-EGFP-bGHpolyA

Plasmid

#221453

Purpose

EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end

Depositor

Omar Akbari

Insert

eGFP

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203C-CMV-5’+3'DR-EGFP-bGHpolyA

Plasmid

#221454

Purpose

EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end

Depositor

Omar Akbari

Insert

eGFP

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203Q-5'dmDR-NT(22)-GFP-bGHpolyA

Plasmid

#221538

Purpose

EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' end

Depositor

Omar Akbari

Insert

eGFP

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA

Plasmid

#221539

Purpose

EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' end

Depositor

Omar Akbari

Insert

eGFP

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203M-CMV-dDiCas7-11-bGHpolyA

Plasmid

#220957

Purpose

Deactivated DiCas7-11 effector with CMV promoter/enhancer

Depositor

Omar Akbari

Insert

DiCas7-11

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203P-CMV-Cas7.1-bGHpolyA

Plasmid

#220960

Purpose

Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector)

Depositor

Omar Akbari

Insert

Truncated DiCas7-11

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDest-pol2-Smarcd1-Myc

Plasmid

#227716

Purpose

Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

Depositor

Kent Hunter

Article

Ross et al Commun Biol. 2024 Oct 10;7(1):1299. doi:

Insert

Smarcd1 (Smarcd1 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

pol2

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:Apollo-NADP+ mVenus

Plasmid

#210518

Purpose

Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Promoter

Zebrafish insulin promoter

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-449 POLH

Plasmid

#201583

Purpose

Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

POLH (POLH Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-449 of DNA polymerase eta

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-542 POLK

Plasmid

#201585

Purpose

Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-542 of DNA polymerase kap…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-ChroME2s-mCerulean

Plasmid

#192582

Purpose

AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-ChroME2f-mCerulean

Plasmid

#192581

Purpose

AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(T304I)

Plasmid

#177133

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-FlucV5His6-PolyA-HhR_AB

Plasmid

#148303

Purpose

Insect Expression of PolyA-HhR

Depositor

Elisa Izaurralde

Insert

PolyA-HhR

Expression

Insect

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 440 of 9867 results

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