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Plasmid#123567PurposeMoChlo kitDepositorInsertLux-E codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-B codon opt. Nt chloroplasts
Plasmid#123566PurposeMoChlo kitDepositorInsertLux-B codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-A codon opt. Nt chloroplasts
Plasmid#123565PurposeMoChlo kitDepositorInsertLux-A codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-D codon opt. Nt chloroplasts
Plasmid#123564PurposeMoChlo kitDepositorInsertLux-D codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-C codon opt. Nt chloroplasts
Plasmid#123563PurposeMoChlo kitDepositorInsertLux-C codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbA-rbcL chimeric 5'UTR (R'SLWT)
Plasmid#123543PurposeMoChlo kitDepositorInsertpsbA-rbcL chimeric 5'UTR (R'SLWT)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)
Plasmid#123542PurposeMoChlo kitDepositorInsertpsbA-rbcL chimeric 5'UTR (R delta 5'SL)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbH 5'UTR + start codon + 1AA
Plasmid#123537PurposeMoChlo kitDepositorInsertpsbH 5'UTR + start codon + 1AA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mCCUC (-20 to -10 deletion)
Plasmid#123536PurposeMoChlo kitDepositorInsertrps2 5'UTR mCCUC (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)
Plasmid#123535PurposeMoChlo kitDepositorInsertrps2 5'UTR mAAGA (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR TCR processed -90 (functional)
Plasmid#123523PurposeMoChlo kitDepositorInsertatpB 5'UTR TCR processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR +10 processed -90 (functional)
Plasmid#123522PurposeMoChlo kitDepositorInsertatpB 5'UTR +10 processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-mPrrn (5-fold decrease transcription)
Plasmid#123478PurposeMoChlo kitDepositorInsertmPrrn (5-fold decrease transcription)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (PEP functional NEP disabled)
Plasmid#123469PurposeMoChlo kitDepositorInsertPatpB (PEP functional NEP disabled)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (PEP and NEP functional)
Plasmid#123468PurposeMoChlo kitDepositorInsertPatpB (PEP and NEP functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (NEP functional PEP disabled)
Plasmid#123466PurposeMoChlo kitDepositorInsertPatpB (NEP functional PEP disabled)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-PA PdxB
Plasmid#12668DepositorInsertErythronate-4-phosphate dehydrogenase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGD283
Plasmid#129733PurposeDestination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
4xCSL-luciferase
Plasmid#41726DepositorInsert4xCSL binding sites
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains a multimerized high affinity CSL bindingā¦PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRL277
Plasmid#70255PurposesacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9
Plasmid#128160PurposePtet-controlled expression of S. pyogenes Cas9DepositorInsertSp Cas9
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)
Plasmid#225433PurposeTranscriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.DepositorInsert4xCBS:mDFR:Rep/RepA:Tnos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9D10A
Plasmid#128161PurposePtet-controlled expression of S. pyogenes Cas9D10A variantDepositorInsertSp Cas9
UseTagsExpressionBacterialMutationD10APromoterPtetAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL271
Plasmid#70254PurposesacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIS-krPAM1
Plasmid#25456DepositorInsertPAM1 (Pam Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAH01SpR
Plasmid#128159PurposepCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDSDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBN8
Plasmid#86711PurposeCloning plasmid for MosSCI transgenesis in C. elegansDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG-Pcon-Scar
Plasmid#203678PurposepQFT-mNG with constitutively expressed mScarlet-IDepositorInsertsmNeonGreen
mScarlet-I
UseTagsExpressionBacterialMutationPromoterPQJ and Phyb18Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRL623
Plasmid#58494Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRL1342
Plasmid#70691PurposeRSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET WT
Plasmid#218503PurposeRIPLET full length in pcDNA3.1DepositorInsertRIPLET (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-cp6
Plasmid#134938PurposeLow phosphate inducible circular permutation of GFPDepositorInsertGFPcp6
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL25
Plasmid#70253PurposeShuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strainsDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL1383a
Plasmid#70692PurposeRSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMW-attB
Plasmid#61814PurposepUASp plasmid with attB for Drosophila transgenesisDepositorTypeEmpty backboneUseTags6xMycExpressionInsectMutationPromoterUASpAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQF-mNGsacB
Plasmid#203673PurposepQF with mNeonGreen and sacB under control of PQ5DepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGIā¦PromoterPQ5Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only